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. 1966 Nov;101(2):480–487. doi: 10.1042/bj1010480

The effect of calcium ions on the glycolytic activity of Ehrlich ascites-tumour cells

F L Bygrave 1,*
PMCID: PMC1270131  PMID: 6007855

Abstract

1. Added Ca2+ inhibited lactate formation from sugar phosphates by intact Ehrlich ascites-tumour cells. Lactate formation from glucose by these cells was unaffected by added Ca2+. 2. The Ca2+ inhibition of lactate formation by intact cells occurred in the extracellular medium. 3. Intact ascites-tumour cells did not take up Ca2+ in vitro. 4. Glycolysis of sugar phosphates by cell extracts as well as pyruvate formation from 3-phosphoglycerate and phosphoenolpyruvate was inhibited by Ca2+. 5. It was concluded that Ca2+ inhibited the pyruvate-kinase (EC 2.7.1.40) reaction. Further, Ca2+ inhibition of pyruvate kinase could be correlated with the overall inhibition of glycolysis. 6. Concentrations of Ca2+ usually present in Krebs–Ringer buffers, inhibited glycolysis and pyruvate-kinase activity by approx. 50%. 7. The inhibition of glycolysis by added Ca2+ could be partially reversed by K+ and completely reversed by Mg2+ or by stoicheiometric amounts of EDTA. 8. The hypothesis is advanced that the inability of tumour cells to take up Ca2+ is a factor contributing towards their high rate of glycolysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHWELL G., DISCHE Z. Inhibition of the metabolism of nucleated red cells by intracellular ions and its relation to intracellular structural factors. Biochim Biophys Acta. 1950 Jan;4(1-3):276–292. doi: 10.1016/0006-3002(50)90034-5. [DOI] [PubMed] [Google Scholar]
  2. BARTLEY W., DAVIES R. E., KREBS H. A. Active transport in animal tissues and subcellular particles. Proc R Soc Lond B Biol Sci. 1954 Mar 25;142(907):187–196. doi: 10.1098/rspb.1954.0020. [DOI] [PubMed] [Google Scholar]
  3. BOSZORMENYI-NAGY I. Formation of phosphopyruvate from phosphoglycerate in hemolyzed human erythrocytes. J Biol Chem. 1955 Jan;212(1):495–499. [PubMed] [Google Scholar]
  4. COMAN D. R. Reduction in cellular adhesiveness upon contact with a carcinogen. Cancer Res. 1960 Sep;20:1202–1204. [PubMed] [Google Scholar]
  5. CRANE R. K., FIELD R. A., CORI C. F. Studies of tissue permeability. I. The penetration of sugars into the Ehrlich ascites tumor cells. J Biol Chem. 1957 Feb;224(2):649–662. [PubMed] [Google Scholar]
  6. DELUCA H. F., ENGSTROM G. W., RASMUSSEN H. The action of vitamin D and parathyroid hormone in vitro on calcium uptake and release by kidney mitochondria. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1604–1609. doi: 10.1073/pnas.48.9.1604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DICKENS F., WILLIAMSON D. H. Pentose phosphate isomerase and epimerase from animal tissues. Biochem J. 1956 Nov;64(3):567–578. doi: 10.1042/bj0640567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DISCHE Z. Qualitative and quantitative colorimetric determination of heptoses. J Biol Chem. 1953 Oct;204(2):983–997. [PubMed] [Google Scholar]
  9. Dickens F., Greville G. D. The metabolism of normal and tumour tissue: Neutral salt effects. Biochem J. 1935 Jun;29(6):1468–1483. doi: 10.1042/bj0291468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GROBECKER H., KROMPHARDT H., MARIANI H., HEINZ E. UNTERSUCHUNGEN UEBER DEN ELEKTROLYTHAUSHALT DER EHRLICH-ASCITES-TUMORZELLE. Biochem Z. 1963 Jul 26;337:462–476. [PubMed] [Google Scholar]
  11. Geiger A. Glycolysis in cell-free extracts of brain. Biochem J. 1940 Apr;34(4):465–482. doi: 10.1042/bj0340465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HEMPLING H. G. Potassium transport in the Ehrlich mouse ascites tumor cell: evidence for autoinhibition by external potassium. J Cell Comp Physiol. 1962 Dec;60:181–198. doi: 10.1002/jcp.1030600302. [DOI] [PubMed] [Google Scholar]
  13. KACHMAR J. F., BOYER P. D. Kinetic analysis of enzyme reactions. II. The potassium activation and calcium inhibition of pyruvic phosphoferase. J Biol Chem. 1953 Feb;200(2):669–682. [PubMed] [Google Scholar]
  14. KIT S., GRIFFIN A. C. Cellular metabolism and cancer: a review. Cancer Res. 1958 Jul;18(6):621–656. [PubMed] [Google Scholar]
  15. KREBS H. A. Body size and tissue respiration. Biochim Biophys Acta. 1950 Jan;4(1-3):249–269. doi: 10.1016/0006-3002(50)90032-1. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. MALMSTROM B. G. Metal-ion specificity in the activation of enolase. Arch Biochem Biophys. 1955 Oct;58(2):381–397. doi: 10.1016/0003-9861(55)90138-7. [DOI] [PubMed] [Google Scholar]
  18. MCILWAIN H. Phosphates of brain during in vitro metabolism: effects of oxygen, glucose, glutamate, glutamine, and calcium and potassium salts. Biochem J. 1952 Oct;52(2):289–295. doi: 10.1042/bj0520289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MELLANBY J., WILLIAMSON D. H. THE EFFECT OF CALCIUM IONS ON KETONE-BODY PRODUCTION BY RAT-LIVER SLICES. Biochem J. 1963 Sep;88:440–444. doi: 10.1042/bj0880440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. THOMASON D., SCHOFIELD R. Calcium exchanges between cells and environment. I. Ehrlich ascites tumour cells of mice, in vivo. Exp Cell Res. 1959 Feb;16(2):324–334. doi: 10.1016/0014-4827(59)90260-5. [DOI] [PubMed] [Google Scholar]
  21. WARBURG O. On the origin of cancer cells. Science. 1956 Feb 24;123(3191):309–314. doi: 10.1126/science.123.3191.309. [DOI] [PubMed] [Google Scholar]
  22. WU R. Leakage of enzymes from ascites tumor cells. Cancer Res. 1959 Dec;19:1217–1222. [PubMed] [Google Scholar]
  23. WU R., RACKER E. Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells. J Biol Chem. 1959 May;234(5):1029–1035. [PubMed] [Google Scholar]

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