Abstract
1. In a study of the metabolism of l-[35S]methionine in vivo, the labelled sulphur compounds of rat liver and brain were separated first by ion-exchange chromatography into two fractions containing (i) free sulphur amino acids such as methionine, cystathionine, cyst(e)ine and homocyst(e)ine and (ii) glutathione. 2. Two-dimensional paper chromatography with butan-1-ol–acetic acid or propionic acid–water in the first direction and 80% acetone or acetone–ethyl methyl ketone–water in the second direction was found superior to other solvent systems for separating the sulphur amino acids. 3. At 10min. after injection of [35S]methionine only a small part of the 35S was found combined in free methionine or other free sulphur amino acids. 4. Evidence was obtained of the presence of adenosyl[35S]methionine and adenosyl[35S]homocysteine in perchloric acid extracts of rat liver and brain. 5. The trans-sulphuration pathway was active in brain as well as in liver.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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