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. 1980 Sep;31(3):381–392. doi: 10.1016/S0006-3495(80)85066-1

Sensitivity to ultraviolet radiation as a function of DNA content in Escherichia coli B/r.

B V Bronk, D G Walbridge
PMCID: PMC1328797  PMID: 7020785

Abstract

Populations of Escherichia coli B/r A were grown to log phase at various growth rates determined by the richness of the medium. The genome content, G, was calculated from log phase doubling times by means of the Cooper-Helmstetter formula. Cell volumes were measured and found to vary linearly with this genome content. Cells with various DNA contents were prepared for ultraviolet irradiation and plated for dark repair under similar conditions. The resulting logarithmic survival curves were all similar in shape: convex up, with straight line portions having approximately the same slope (D0 = 11.4 +/- 0.2 J/m2). The shoulders however increase in width with calculated DNA content giving an extrapolation number which varies roughly as exp(G) or exp (0.6 Gmax).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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