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. 1982 Apr;38(1):7–13. doi: 10.1016/S0006-3495(82)84524-4

Photon correlation spectroscopic study of the size distribution of phospholipid vesicles.

J Goll, F D Carlson, Y Barenholz, B J Litman, T E Thompson
PMCID: PMC1328807  PMID: 7074200

Abstract

The dependence of phospholipid vesicle size on lipid composition is investigated by photon correlation spectroscopy. For each lipid composition prolonged ultracentrifugation was used to isolate a nearly uniform population of minimum-sized vesicles. The residual size variations in the samples were sufficient to cause polydispersity that made comparisons between samples difficult. Analyses of the data by the method of cumulants and by a method for approximating the particle size distributions directly are presented. The latter method made possible unambiguous comparisons that revealed small but systematic dependences of vesicle size on composition in vesicles containing mixtures of egg phosphatidylcholine and phosphatidylethanolamine, egg phosphatidylcholine and beef brain sphingomyelin, and in single lipid vesicles of egg phosphatidylcholine, dioleylphosphatidylcholine, and beef brain sphingomyelin. These size dependences are quantified within the resolution limits of the technique and their implications are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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