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. 1966 Mar;183(1):179–188. doi: 10.1113/jphysiol.1966.sp007859

The localization of lysosomal enzymes in chromaffin tissue

A D Smith, H Winkler
PMCID: PMC1357535  PMID: 5945247

Abstract

1. An homogenate of bovine adrenal medulla contains significant amounts of six acid hydrolases: acid ribonuclease, acid deoxyribonuclease, cathepsin, acid phosphatase, β-glucuronidase and arylsulphatase. Most of the activity of each enzyme could be sedimented in the large-granule fraction at 242,000 g-min.

2. Differential centrifugation indicated the presence of three populations of particles, which sedimented at slightly different rates; these are, in order of decreasing sedimentation rate, mitochondria, particles containing the acid hydrolases, and chromaffin granules.

3. The three types of particle could be separated by ultracentrifuging the large-granule fraction in a sucrose density gradient. Most of the activity of each hydrolase was recovered in a layer intermediate between those formed by mitochondria and chromaffin granules.

4. The large-granule fraction therefore contains particles which are defined by their enzyme content as lysosomes.

5. Highly purified chromaffin granules, containing less than 5% of the activity of each acid hydrolase, were obtained from the gradient.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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