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. 1988 Dec;26(6):691–695. doi: 10.1111/j.1365-2125.1988.tb05306.x

Human alpha-calcitonin gene-related peptide (CGRP) is a potent vasodilator in human mesenteric vasculature.

I Marshall 1, S J Al-Kazwini 1, J J Holman 1, R K Craig 1
PMCID: PMC1386582  PMID: 3266556

Abstract

1. The effect of human alpha-calcitonin gene-related peptide (CGRP) and sodium nitroprusside have been measured on human isolated mesenteric vasculature and on rings of human superior mesenteric artery and saphenous vein. 2. When noradrenaline (10(-5) M) was used as the vasoconstrictor in preparations perfused with Krebs solution at constant flow, human alpha-CGRP was 10 times more potent than sodium nitroprusside in evoking dose-dependent falls in perfusion pressure. 3. Human alpha-CGRP and sodium nitroprusside were about equipotent at relaxing rings of superior mesenteric artery contracted by noradrenaline (10(-6) M). When the tone of saphenous vein rings was raised by noradrenaline (10(-6) M), human alpha-CGRP did not relax the vascular smooth muscle. 4. The results show that human alpha-CGRP is a potent vasodilator in human arterial preparations and may act preferentially on arterioles rather than large arteries.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Kazwini S. J., Craig R. K., Marshall I. Postjunctional inhibition of contractor responses in the mouse vas deferens by rat and human calcitonin gene-related peptides (CGRP). Br J Pharmacol. 1986 May;88(1):173–180. doi: 10.1111/j.1476-5381.1986.tb09484.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brain S. D., Williams T. J., Tippins J. R., Morris H. R., MacIntyre I. Calcitonin gene-related peptide is a potent vasodilator. Nature. 1985 Jan 3;313(5997):54–56. doi: 10.1038/313054a0. [DOI] [PubMed] [Google Scholar]
  3. Edbrooke M. R., Parker D., McVey J. H., Riley J. H., Sorenson G. D., Pettengill O. S., Craig R. K. Expression of the human calcitonin/CGRP gene in lung and thyroid carcinoma. EMBO J. 1985 Mar;4(3):715–724. doi: 10.1002/j.1460-2075.1985.tb03688.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gennari C., Fischer J. A. Cardiovascular action of calcitonin gene-related peptide in humans. Calcif Tissue Int. 1985 Dec;37(6):581–584. doi: 10.1007/BF02554909. [DOI] [PubMed] [Google Scholar]
  5. Girgis S. I., Macdonald D. W., Stevenson J. C., Bevis P. J., Lynch C., Wimalawansa S. J., Self C. H., Morris H. R., MacIntyre I. Calcitonin gene-related peptide: potent vasodilator and major product of calcitonin gene. Lancet. 1985 Jul 6;2(8445):14–16. doi: 10.1016/s0140-6736(85)90059-5. [DOI] [PubMed] [Google Scholar]
  6. Hanko J., Hardebo J. E., Kåhrström J., Owman C., Sundler F. Calcitonin gene-related peptide is present in mammalian cerebrovascular nerve fibres and dilates pial and peripheral arteries. Neurosci Lett. 1985 Jun 4;57(1):91–95. doi: 10.1016/0304-3940(85)90045-x. [DOI] [PubMed] [Google Scholar]
  7. Holman J. J., Craig R. K., Marshall I. Human alpha- and beta-CGRP and rat alpha-CGRP are coronary vasodilators in the rat. Peptides. 1986 Mar-Apr;7(2):231–235. doi: 10.1016/0196-9781(86)90218-4. [DOI] [PubMed] [Google Scholar]
  8. Jonas V., Lin C. R., Kawashima E., Semon D., Swanson L. W., Mermod J. J., Evans R. M., Rosenfeld M. G. Alternative RNA processing events in human calcitonin/calcitonin gene-related peptide gene expression. Proc Natl Acad Sci U S A. 1985 Apr;82(7):1994–1998. doi: 10.1073/pnas.82.7.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kraenzlin M. E., Ch'ng J. L., Mulderry P. K., Ghatei M. A., Bloom S. R. Infusion of a novel peptide, calcitonin gene-related peptide (CGRP) in man. Pharmacokinetics and effects on gastric acid secretion and on gastrointestinal hormones. Regul Pept. 1985 Mar;10(2-3):189–197. doi: 10.1016/0167-0115(85)90013-8. [DOI] [PubMed] [Google Scholar]
  10. Kubota M., Moseley J. M., Butera L., Dusting G. J., MacDonald P. S., Martin T. J. Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cells. Biochem Biophys Res Commun. 1985 Oct 15;132(1):88–94. doi: 10.1016/0006-291x(85)90992-1. [DOI] [PubMed] [Google Scholar]
  11. Lenz H. J., Rivier J. E., Brown M. R. Biological actions of human and rat calcitonin and calcitonin gene-related peptide. Regul Pept. 1985 Oct;12(2):81–89. doi: 10.1016/0167-0115(85)90189-2. [DOI] [PubMed] [Google Scholar]
  12. Marshall I., Al-Kazwini S. J., Holman J. J., Craig R. K. Human and rat alpha-CGRP but not calcitonin cause mesenteric vasodilatation in rats. Eur J Pharmacol. 1986 Apr 16;123(2):217–222. doi: 10.1016/0014-2999(86)90662-x. [DOI] [PubMed] [Google Scholar]
  13. Marshall I., Al-Kazwini S. J., Roberts P. M., Shepperson N. B., Adams M., Craig R. K. Cardiovascular effects of human and rat CGRP compared in the rat and other species. Eur J Pharmacol. 1986 Apr 16;123(2):207–216. doi: 10.1016/0014-2999(86)90661-8. [DOI] [PubMed] [Google Scholar]
  14. McEwan J., Larkin S., Davies G., Chierchia S., Brown M., Stevenson J., MacIntyre I., Maseri A. Calcitonin gene-related peptide: a potent dilator of human epicardial coronary arteries. Circulation. 1986 Dec;74(6):1243–1247. doi: 10.1161/01.cir.74.6.1243. [DOI] [PubMed] [Google Scholar]
  15. Morris H. R., Panico M., Etienne T., Tippins J., Girgis S. I., MacIntyre I. Isolation and characterization of human calcitonin gene-related peptide. Nature. 1984 Apr 19;308(5961):746–748. doi: 10.1038/308746a0. [DOI] [PubMed] [Google Scholar]
  16. Mulderry P. K., Ghatei M. A., Rodrigo J., Allen J. M., Rosenfeld M. G., Polak J. M., Bloom S. R. Calcitonin gene-related peptide in cardiovascular tissues of the rat. Neuroscience. 1985 Mar;14(3):947–954. doi: 10.1016/0306-4522(85)90156-3. [DOI] [PubMed] [Google Scholar]
  17. Rosenfeld M. G., Amara S. G., Evans R. M. Alternative RNA processing: determining neuronal phenotype. Science. 1984 Sep 21;225(4668):1315–1320. doi: 10.1126/science.6089345. [DOI] [PubMed] [Google Scholar]
  18. Rosenfeld M. G., Mermod J. J., Amara S. G., Swanson L. W., Sawchenko P. E., Rivier J., Vale W. W., Evans R. M. Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature. 1983 Jul 14;304(5922):129–135. doi: 10.1038/304129a0. [DOI] [PubMed] [Google Scholar]
  19. Sasaki Y., Hayashi N., Kasahara A., Matsuda H., Fusamoto H., Sato N., Hillyard C. J., Girgis S., MacIntyre I., Emson P. C. Calcitonin gene-related peptide in the hepatic and splanchnic vascular systems of the rat. Hepatology. 1986 Jul-Aug;6(4):676–681. doi: 10.1002/hep.1840060423. [DOI] [PubMed] [Google Scholar]
  20. Schifter S., Williams E. D., Craig R. K., Hansen H. H. Calcitonin gene-related peptide and calcitonin in medullary thyroid carcinoma. Clin Endocrinol (Oxf) 1986 Dec;25(6):703–710. doi: 10.1111/j.1365-2265.1986.tb03626.x. [DOI] [PubMed] [Google Scholar]
  21. Sigrist S., Franco-Cereceda A., Muff R., Henke H., Lundberg J. M., Fischer J. A. Specific receptor and cardiovascular effects of calcitonin gene-related peptide. Endocrinology. 1986 Jul;119(1):381–389. doi: 10.1210/endo-119-1-381. [DOI] [PubMed] [Google Scholar]
  22. Skofitsch G., Jacobowitz D. M. Calcitonin gene-related peptide: detailed immunohistochemical distribution in the central nervous system. Peptides. 1985 Jul-Aug;6(4):721–745. doi: 10.1016/0196-9781(85)90178-0. [DOI] [PubMed] [Google Scholar]
  23. Skofitsch G., Jacobowitz D. M. Quantitative distribution of calcitonin gene-related peptide in the rat central nervous system. Peptides. 1985 Nov-Dec;6(6):1069–1073. doi: 10.1016/0196-9781(85)90429-2. [DOI] [PubMed] [Google Scholar]
  24. Steenbergh P. H., Höppener J. W., Zandberg J., Lips C. J., Jansz H. S. A second human calcitonin/CGRP gene. FEBS Lett. 1985 Apr 22;183(2):403–407. doi: 10.1016/0014-5793(85)80820-6. [DOI] [PubMed] [Google Scholar]
  25. Steenbergh P. H., Höppener J. W., Zandberg J., Van de Ven W. J., Jansz H. S., Lips C. J. Calcitonin gene related peptide coding sequence is conserved in the human genome and is expressed in medullary thyroid carcinoma. J Clin Endocrinol Metab. 1984 Aug;59(2):358–360. doi: 10.1210/jcem-59-2-358. [DOI] [PubMed] [Google Scholar]
  26. Struthers A. D., Brown M. J., Macdonald D. W., Beacham J. L., Stevenson J. C., Morris H. R., MacIntyre I. Human calcitonin gene related peptide: a potent endogenous vasodilator in man. Clin Sci (Lond) 1986 Apr;70(4):389–393. doi: 10.1042/cs0700389. [DOI] [PubMed] [Google Scholar]
  27. Thom S. M., Hughes A. D., Goldberg P., Martin G., Schachter M., Sever P. S. The actions of calcitonin gene related peptide and vasoactive intestinal peptide as vasodilators in man in vivo and in vitro. Br J Clin Pharmacol. 1987 Aug;24(2):139–144. doi: 10.1111/j.1365-2125.1987.tb03154.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Uddman R., Edvinsson L., Ekblad E., Håkanson R., Sundler F. Calcitonin gene-related peptide (CGRP): perivascular distribution and vasodilatory effects. Regul Pept. 1986 Aug;15(1):1–23. doi: 10.1016/0167-0115(86)90071-6. [DOI] [PubMed] [Google Scholar]
  29. Wharton J., Gulbenkian S., Mulderry P. K., Ghatei M. A., McGregor G. P., Bloom S. R., Polak J. M. Capsaicin induces a depletion of calcitonin gene-related peptide (CGRP)-immunoreactive nerves in the cardiovascular system of the guinea pig and rat. J Auton Nerv Syst. 1986 Aug;16(4):289–309. doi: 10.1016/0165-1838(86)90035-4. [DOI] [PubMed] [Google Scholar]

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