Abstract
Five sialic acid variants have been demonstrated in normal, 'transitional', and tumour extracts of human large bowel mucosa by thin-layer chromatography. Two-dimensional thin-layer chromatography, with intermediate alkaline hydrolysis, confirmed that the variants were N-acetyl neuraminic acids with additional O-acylation. Cases studied so far show changes in the proportions of these variants in malignancy.
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- BOHM P., DAUBER S., BAUMEISTER L. Uber Neuraminsäure, ihr Vorkommen und ihre Bestimmung im Serum. Klin Wochenschr. 1954 Apr 1;32(13-14):289–292. doi: 10.1007/BF01467396. [DOI] [PubMed] [Google Scholar]
- Buscher H. P., Casals-Stenzel J., Schaufer R. New sialic acids. Identification of N-glycoloyl-O-acetylneuraminic acids and N-acetyl-O-glycoloylneuraminic acids by improved methods for detection of N-acyl and O-acyl groups and by gas-liquid chromatography. Eur J Biochem. 1974 Dec 16;50(1):71–82. doi: 10.1111/j.1432-1033.1974.tb03873.x. [DOI] [PubMed] [Google Scholar]
- Filipe M. I., Branfoot A. C. Mucin histochemistry of the colon. Curr Top Pathol. 1976;63:143–178. doi: 10.1007/978-3-642-66481-6_5. [DOI] [PubMed] [Google Scholar]
- Filipe M. I., Cooke K. B. Changes in composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with the normal: a biochemical investigation. J Clin Pathol. 1974 Apr;27(4):315–318. doi: 10.1136/jcp.27.4.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Filipe M. I. The value of a study of the mucosubstances in rectal biopsies from patients with carcinoma of the rectum and lower sigmoid in the diagnosis of premalignant mucosa. J Clin Pathol. 1972 Feb;25(2):123–128. doi: 10.1136/jcp.25.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Filipe M. I. Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum. Gut. 1969 Jul;10(7):577–586. doi: 10.1136/gut.10.7.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gold D. V., Miller F. Characterization of human colonic mucoprotein antigen. Immunochemistry. 1974 Jul;11(7):369–375. doi: 10.1016/0019-2791(74)90190-6. [DOI] [PubMed] [Google Scholar]
- Lipkin M., Deschner E., Troncale F. Cell differentiation and the development of colonic neoplasms. Gastroenterology. 1970 Aug;59(2):303–307. [PubMed] [Google Scholar]
- Reid P. E., Culling C. F., Tsang W. C., Clay M. G., Ramey C. W., Dunn W. L. The demonstration of O-acetylated sialic acids in colonic epithelial glycoproteins. Can J Biochem. 1975 Mar;53(3):388–391. doi: 10.1139/o75-053. [DOI] [PubMed] [Google Scholar]