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. 1966 Aug;11(2):155–162.

Basement membrane specific antisera produced to solubilized tissue fractions

J Myers, J V Frei, J J Cohen, B Rose, M Richter
PMCID: PMC1423765  PMID: 4958573

Abstract

Attempts were made to produce antisera to solubilized tissue fractions rich in basement membranes and reticulin. Murine tissue fractions solubilized with sodium hydroxide elicited precipitating antibodies upon injection into rabbits. Although no nephrotoxic effect was observed upon injecting the rabbit antisera into mice, the antisera were fixed to the glomerular basement membrane, and not elsewhere, within 5 minutes of injection and remained fixed for at least 3 weeks.

Specificity studies suggested that in addition to unique antigens, reticulin and epithelial basement membranes share a common antigen which is responsible for the similar in vitro immunofluorescence produced by antisera to tissue fractions rich in one or the other of these components.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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