Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1970 Aug;19(2):329–342.

The interaction of normal lymphocytes and cells from lymphoid cell lines

I. The nature of the activation process

D A Hardy, Stella Knight, N R Ling
PMCID: PMC1455751  PMID: 4990403

Abstract

Human peripheral lymphocytes from all donors tested were mitotically activated when cultured in the presence of X-irradiated cells from continuously cultivated lymphoid cell lines (LCL). X-irradiated cells from LCL originating from normal individuals were effective as well as those of Burkitt's tumour origin and cells from LCL reported to be free of herpes-like virus were as effective as those reported to contain virus. The activation was of much greater intensity than the normal mixed lymphocyte reaction but occurred over a similar culture period. The reaction occurred only under conditions permitting direct cell contact between the two populations of cells. Most stimulation was obtained with viable X-irradiated LCL cells. Disrupted LCL cells were ineffective.

Full text

PDF
329

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bach F. H. Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity. J Exp Med. 1968 Oct 1;128(4):623–637. doi: 10.1084/jem.128.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beierle J. W. Cell proliferation: enhancement by extracts from cell surfaces of polyoma-virus-transformed cells. Science. 1968 Aug 23;161(3843):798–799. doi: 10.1126/science.161.3843.798. [DOI] [PubMed] [Google Scholar]
  3. COULSON A. S., CHALMERS D. G. SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD. Lancet. 1964 Feb 29;1(7331):468–469. doi: 10.1016/s0140-6736(64)90799-8. [DOI] [PubMed] [Google Scholar]
  4. Diehl V., Henle G., Henle W., Kohn G. Demonstration of a herpes group virus in cultures of peripheral leukocytes from patients with infectious mononucleosis. J Virol. 1968 Jul;2(7):663–669. doi: 10.1128/jvi.2.7.663-669.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EPSTEIN M. A., BARR Y. M., ACHONG B. G. A SECOND VIRUS-CARRYING TISSUE CULTURE STRAIN (EB2) OF LYMPHOBLASTS FROM BURKITT'S LYMPHOMA. Pathol Biol. 1964 Dec;12:1233–1234. [PubMed] [Google Scholar]
  6. Gerber P., Hamre D., Moy R. A., Rosenblum E. N. Infectious mononucleosis: complement-fixing antibodies to herpes-like virus associated with Burkitt lymphoma. Science. 1968 Jul 12;161(3837):173–175. doi: 10.1126/science.161.3837.173. [DOI] [PubMed] [Google Scholar]
  7. Gerber P., Monroe J. H. Studies on leukocytes growing in continuous culture derived from normal human donors. J Natl Cancer Inst. 1968 Apr;40(4):855–866. [PubMed] [Google Scholar]
  8. Hardy D. A., Ling N. R. Effects of some cellular antigens on lymphocytes and the nature of the mixed lymphocyte reaction. Nature. 1969 Feb 8;221(5180):545–548. doi: 10.1038/221545a0. [DOI] [PubMed] [Google Scholar]
  9. Hardy D. A., Ling N. R., Knight S. C. Interaction between peripheral blood leucocytes and x-irradiated cells from lymphoid cell lines. Experientia. 1969 Apr 15;25(4):404–406. doi: 10.1007/BF01899949. [DOI] [PubMed] [Google Scholar]
  10. Henle G., Henle W., Diehl V. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc Natl Acad Sci U S A. 1968 Jan;59(1):94–101. doi: 10.1073/pnas.59.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henle G., Henle W. Immunofluorescence, interference, and complement fixation technics in the detection of the herpes-type virus in Burkitt tumor cell lines. Cancer Res. 1967 Dec;27(12):2442–2446. [PubMed] [Google Scholar]
  12. Henle W., Diehl V., Kohn G., Zur Hausen H., Henle G. Herpes-type virus and chromosome marker in normal leukocytes after growth with irradiated Burkitt cells. Science. 1967 Sep 1;157(3792):1064–1065. doi: 10.1126/science.157.3792.1064. [DOI] [PubMed] [Google Scholar]
  13. Herberman R. B., Fahey J. L. Cytotoxic antibody in Burkitt's tumor and normal human serum reactive with cultures of lymphoid cells. Proc Soc Exp Biol Med. 1968 Mar;127(3):938–940. doi: 10.3181/00379727-127-32839. [DOI] [PubMed] [Google Scholar]
  14. Imrie R. C., Mueller G. C. Release of a lymphocyte growth promoter in leucocyte cultures. Nature. 1968 Sep 21;219(5160):1277–1279. doi: 10.1038/2191277a0. [DOI] [PubMed] [Google Scholar]
  15. Kasakura S., Lowenstein L. Nature of the blastogenic material in the medium from peripheral leukocyte cultures. Transplantation. 1967 May;5(3):459–464. doi: 10.1097/00007890-196705000-00008. [DOI] [PubMed] [Google Scholar]
  16. Kasakura S., Lowenstein L. The factors affecting the strength of "one-way" stimulation with irradiated leukocytes in mixed leukocyte cultures. J Immunol. 1968 Jul;101(1):12–17. [PubMed] [Google Scholar]
  17. Klein G., Pearson G., Nadkarni J. S., Nadkarni J. J., Klein E., Henle G., Henle W., Clifford P. Relation between Epstein-Barr viral and cell membrane immunofluorescence of Burkitt tumor cells. I. Dependence of cell membrane immunofluorescence on presence of EB virus. J Exp Med. 1968 Nov 1;128(5):1011–1020. doi: 10.1084/jem.128.5.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LING N. R., SPICER E., JAMES K., WILLIAMSON N. THE ACTIVATION OF HUMAN PERIPHERAL LYMPHOCYTES BY PRODUCTS OF STAPHYLOCOCCI. Br J Haematol. 1965 Jul;11:421–431. doi: 10.1111/j.1365-2141.1965.tb06604.x. [DOI] [PubMed] [Google Scholar]
  19. LING N. R. The attachment of proteins to aldehyde-tanned cells. Br J Haematol. 1961 Jul;7:299–302. doi: 10.1111/j.1365-2141.1961.tb00340.x. [DOI] [PubMed] [Google Scholar]
  20. Moore G. E., McLimans W. F. The life span of the cultured normal cell: concepts derived from studies of human lymphoblasts. J Theor Biol. 1968 Aug;20(2):217–226. doi: 10.1016/0022-5193(68)90191-4. [DOI] [PubMed] [Google Scholar]
  21. Moorhead J. F., Connolly J. J., McFarland W. Factors affecting the reactivity of human lymphocytes in vitro. I. Cell number, duration of culture and surface area. J Immunol. 1967 Aug;99(2):413–419. [PubMed] [Google Scholar]
  22. Old L. J., Boyse E. A., Oettgen H. F., Harven E. D., Geering G., Williamson B., Clifford P. Precipitating antibody in human serum to an antigen present in cultured burkitt's lymphoma cells. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1699–1704. doi: 10.1073/pnas.56.6.1699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pope J. H. Establishment of cell lines from peripheral leucocytes in infectious mononucleosis. Nature. 1967 Nov 25;216(5117):810–811. doi: 10.1038/216810a0. [DOI] [PubMed] [Google Scholar]
  24. Pope J. H., Horne M. K., Scott W. Transformation of foetal human keukocytes in vitro by filtrates of a human leukaemic cell line containing herpes-like virus. Int J Cancer. 1968 Nov 15;3(6):857–866. doi: 10.1002/ijc.2910030619. [DOI] [PubMed] [Google Scholar]
  25. Schellekens P. T., Vriesendorp B., Eijsvoogel V. P., Van Leeuwen A., Van Rood J. J., Miggiano V., Ceppellini R. Lymphocyte transformation in vitro. II. Mixed lymphocyte culture in relation to leucocyte antigens. Clin Exp Immunol. 1970 Feb;6(2):241–254. [PMC free article] [PubMed] [Google Scholar]
  26. Schrek R. Radioresistance as a measure of interaction of human lymphocytes. Arch Pathol. 1968 Jan;85(1):31–35. [PubMed] [Google Scholar]
  27. Schwarz M. R. The mixed lymphocyte reaction: an in vitro test for tolerance. J Exp Med. 1968 May 1;127(5):879–890. doi: 10.1084/jem.127.5.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wilson D. B. Quantitative studies on the mixed lymphocyte interaction in rats. I. Conditions and parameters of response. J Exp Med. 1967 Oct 1;126(4):625–654. doi: 10.1084/jem.126.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES