Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1980 Nov;41(3):653–662.

Activation of secondary cytotoxic lymphocytes by cell-free factors from I-region-primed and D-region-primed lymphocytes.

S Kano, K Oshimi, M Sumiya, N Gonda
PMCID: PMC1458130  PMID: 6161875

Abstract

Cell co-operation in the generation of secondary cytotoxic responses was studied by selectively sensitizing lymphocytes in mixed lymphocyte culture (MLC) across I or D region difference and by combining the primed lymphocytes in the secondary MLC. Secondary cytotoxic responses were induced in D-region-primed lymphocytes by restimulation with the original priming D-region antigens, by co-culturing with the I-region-primed lymphocytes in the presence of the priming I-region antigens, or by cell-free supernatants obtained 24 h after the restimulation of D-region-primed lymphocytes and I-region-primed lymphocytes, The active MLC supernatants produced by both I-region-primed and D-region-primed cells also induced accelerated proliferative responses in D-region-primed lymphocytes. Heat-treatment or ultraviolet irradiation of the stimulator cells eliminated the capacity of the cells to induce the production of CTL-helper factor in I-region-primed and D-region-primed lymphocytes. It was concluded that both I-region-primed and D-region-primed lymphocytes produce a cell-free factor which induces proliferation and secondary cytotoxicity in D-region-primed lymphocytes. The possible participation of D-region reactive helper T cells and D-region reactive cytotoxic T cells in the cytotoxic responses to D-region antigens in the absence of I-region difference is discussed.

Full text

PDF
662

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alter B. J., Grillot-Courvalin C., Bach M. L., Zier K. S., Sondel P. M., Bach F. H. Secondary cell-mediated lympholysis: importance of H-2 LD and SD factors. J Exp Med. 1976 May 1;143(5):1005–1014. doi: 10.1084/jem.143.5.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alter B. J., Schendel D. J., Bach M. L., Bach F. H., Klein J., Stimpfling J. H. Cell-mediated lympholysis. Importance of serologically defined H-2 regions. J Exp Med. 1973 May 1;137(5):1303–1309. doi: 10.1084/jem.137.5.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fyfe D. A., Finke J. H. Soluble helper factor(s) participates in the generation of cell mediated cytotoxicity directed against syngeneic tumor cells. J Immunol. 1979 Mar;122(3):1156–1161. [PubMed] [Google Scholar]
  5. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  6. Gillis S., Smith K. A. Long term culture of tumour-specific cytotoxic T cells. Nature. 1977 Jul 14;268(5616):154–156. doi: 10.1038/268154a0. [DOI] [PubMed] [Google Scholar]
  7. Glaser M. T-T cell synergy in the in vitro generation of secondary cell-mediated cytotoxicity against syngeneic SV-40 transformed cells. J Immunol. 1979 Mar;122(3):973–979. [PubMed] [Google Scholar]
  8. Lafferty K. J., Misko I. S., Cooley M. A. Allogeneic stimulation modulates the in vitro response of T cells to transplantation antigen. Nature. 1974 May 17;249(454):275–276. doi: 10.1038/249275a0. [DOI] [PubMed] [Google Scholar]
  9. MacDonald H. R., Sordat B., Cerottini J. C., Brunner K. T. Generation of cytotoxic T lymphocytes in vitro. IV. Functional activation of memory cells in the absence of DNA synthesis. J Exp Med. 1975 Sep 1;142(3):622–636. doi: 10.1084/jem.142.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Melief C. J., van der Meulen M. Y., Christiaans B. J., de Greeve P. Cooperation between subclass of T lymphocytes in the in vitro generation of cytotoxicity against a mutant H-2K difference. An analysis with anti-Lyt antisera. Eur J Immunol. 1979 Jan;9(1):7–12. doi: 10.1002/eji.1830090103. [DOI] [PubMed] [Google Scholar]
  11. Morgan D. A., Ruscetti F. W., Gallo R. Selective in vitro growth of T lymphocytes from normal human bone marrows. Science. 1976 Sep 10;193(4257):1007–1008. doi: 10.1126/science.181845. [DOI] [PubMed] [Google Scholar]
  12. Nabholz M., Vives J., Young H. M., Meo T., Miggiano V., Rijnbeek A., Shreffler D. C. Cell-mediated cell lysis in vitro: genetic control of killer cell production and target specificities in the mouse. Eur J Immunol. 1974 May;4(5):378–387. doi: 10.1002/eji.1830040514. [DOI] [PubMed] [Google Scholar]
  13. Panfili P. R., Dutton R. W. Alloantigen-induced T helper activity. I. Minimal genetic differences necessary to induce a positive allogeneic effect. J Immunol. 1978 Jun;120(6):1897–1901. [PubMed] [Google Scholar]
  14. Plate J. M. Soluble factors substitute for T-T-cell collaboration in generation of T-killer lymphocytes. Nature. 1976 Mar 25;260(5549):329–331. doi: 10.1038/260329a0. [DOI] [PubMed] [Google Scholar]
  15. Revised nomenclature for antigen-nonspecific T cell proliferation and helper factors. J Immunol. 1979 Dec;123(6):2928–2929. [PubMed] [Google Scholar]
  16. Rosenberg S. A., Spiess P. J., Schwarz S. In vitro growth of murine T cells. I. Production of factors necessary for T cell growth. J Immunol. 1978 Nov;121(5):1946–1950. [PubMed] [Google Scholar]
  17. Ryser J. E., Cerottini J. C., Brunner K. T. Generation of cytolytic T lymphocytes in vitro. IX. induction of secondary CTL responses in primary long-term MLC by supernatants from secondary MLC. J Immunol. 1978 Feb;120(2):370–377. [PubMed] [Google Scholar]
  18. Strausser J. L., Rosenberg S. A. In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens. J Immunol. 1978 Oct;121(4):1491–1495. [PubMed] [Google Scholar]
  19. Swain S. L., Panfili P. R. Helper cells activated by allogeneic H-2K or H-2D differences have a Ly phenotype distinct from those responsive to I differences. J Immunol. 1979 Feb;122(2):383–391. [PubMed] [Google Scholar]
  20. Uotila M., Rode H. N., Gordon J. Blastogenic factor: its role in the mixed leukocyte culture reaction. Eur J Immunol. 1978 Feb;8(2):133–138. doi: 10.1002/eji.1830080212. [DOI] [PubMed] [Google Scholar]
  21. Wagner H., Röllinghoff M. T-T-cell interactions during the vitro cytotoxic allograft responses. I. Soluble products from activated Lyl+ T cells trigger autonomously antigen-primed Ly23+ T cells to cell proliferation and cytolytic activity. J Exp Med. 1978 Dec 1;148(6):1523–1538. doi: 10.1084/jem.148.6.1523. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES