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. 1978 Dec;24(3):645–656. doi: 10.1016/S0006-3495(78)85410-1

Double-strand breaks from single photochemical events in DNA containing 5-bromouracil.

F Krasin, F Hutchinson
PMCID: PMC1473508  PMID: 367461

Abstract

Ultraviolet irradiation of Escherichia coli cells with a low level of 5-bromouracil incorporated produces DNA double-strand breaks by single photochemical events, one such break per 100 single-strand breaks, the latter assayed in alkali-denatured DNA. About 2.5--4 double-strand breaks are produced per "lethal hit," compared with about 6 double-strand breaks per lethal hit induced by gamma rays. These results are consistent with the hypothesis that an unrepaired DNA double-strand break is a major lethal event in both cases. The increase in sensitivity to ultraviolet (measured by colony-forming ability) seems linear in the number of bromouracils incorporated (0--20% of the thymines), and the linear relationship is much the same for incorporation in one or in both strands of the DNA double helix.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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