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. 1991 Dec;86(3):483–488. doi: 10.1111/j.1365-2249.1991.tb02957.x

Autoantibodies against Forssman glycolipids in Graves' disease and Hashimoto's thyroiditis.

T Ariga 1, T Yoshida 1, T Mimori 1, R K Yu 1
PMCID: PMC1554196  PMID: 1747956

Abstract

Sera from patients with Graves' disease and Hashimoto's thyroiditis have been shown to react with the Forssman glycolipid antigen (Gb5) using the techniques of high performance thin-layer chromatography (HPTLC) immunostaining and ELISA. Human monoclonal antibodies (MoAbs) have been prepared by fusion of human myeloma with peripheral lymphocytes from patients with Graves' disease. A MoAb, TRMo-4, reacted strongly and specifically with Gb5. These results suggest that anti-Forssman antibody may be involved in the pathogenesis of these autoimmune diseases. The detection of anti-Forssman glycolipid antibody may provide a useful means for clinical diagnosis and therapy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ariga T., Kusunoki S., Asano K., Oshima M., Asano M., Mannen T., Yu R. K. Localization of sulfated glucuronyl glycolipids in human dorsal root and sympathetic ganglia. Brain Res. 1990 Jun 11;519(1-2):57–64. doi: 10.1016/0006-8993(90)90060-o. [DOI] [PubMed] [Google Scholar]
  2. Ariga T., Macala L. J., Saito M., Margolis R. K., Greene L. A., Margolis R. U., Yu R. K. Lipid composition of PC12 pheochromocytoma cells: characterization of globoside as a major neutral glycolipid. Biochemistry. 1988 Jan 12;27(1):52–58. doi: 10.1021/bi00401a010. [DOI] [PubMed] [Google Scholar]
  3. Ariga T., Murata T., Oshima M., Maezawa M., Miyatake T. Characterization of glycosphingolipids by direct inlet chemical ionization mass spectrometry. J Lipid Res. 1980 Sep;21(7):879–887. [PubMed] [Google Scholar]
  4. Arnon R., Crisp E., Kelley R., Ellison G. W., Myers L. W., Tourtellotte W. W. Anti-ganglioside antibodies in multiple sclerosis. J Neurol Sci. 1980 May;46(2):179–186. doi: 10.1016/0022-510x(80)90076-3. [DOI] [PubMed] [Google Scholar]
  5. Edwards P. A., Smith C. M., Neville A. M., O'Hare M. J. A human-hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line. Eur J Immunol. 1982 Aug;12(8):641–648. doi: 10.1002/eji.1830120804. [DOI] [PubMed] [Google Scholar]
  6. Endo T., Scott D. D., Stewart S. S., Kundu S. K., Marcus D. M. Antibodies to glycosphingolipids in patients with multiple sclerosis and SLE. J Immunol. 1984 Apr;132(4):1793–1797. [PubMed] [Google Scholar]
  7. Feizi T. Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature. 1985 Mar 7;314(6006):53–57. doi: 10.1038/314053a0. [DOI] [PubMed] [Google Scholar]
  8. Feizi T. The blood group Ii system: a carbohydrate antigen system defined by naturally monoclonal or oligoclonal autoantibodies of man. Immunol Commun. 1981;10(2):127–156. doi: 10.3109/08820138109050693. [DOI] [PubMed] [Google Scholar]
  9. Fry J. M., Weissbarth S., Lehrer G. M., Bornstein M. B. Cerebroside antibody inhibits sulfatide synthesis and myelination and demyelinates in cord tissue cultures. Science. 1974 Feb 8;183(4124):540–542. doi: 10.1126/science.183.4124.540. [DOI] [PubMed] [Google Scholar]
  10. Hakomori S. Bifunctional role of glycosphingolipids. Modulators for transmembrane signaling and mediators for cellular interactions. J Biol Chem. 1990 Nov 5;265(31):18713–18716. [PubMed] [Google Scholar]
  11. Hakomori S. Glycosphingolipids in cellular interaction, differentiation, and oncogenesis. Annu Rev Biochem. 1981;50:733–764. doi: 10.1146/annurev.bi.50.070181.003505. [DOI] [PubMed] [Google Scholar]
  12. Hakomori S., Wang S. M., Young W. W., Jr Isoantigenic expression of Forssman glycolipid in human gastric and colonic mucosa: its possible identity with "A-like antigen" in human cancer. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3023–3027. doi: 10.1073/pnas.74.7.3023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hirano T., Hashimoto H., Shiokawa Y., Iwamori M., Nagai Y., Kasai M., Ochiai Y., Okumura K. Antiglycolipid autoantibody detected in the sera from systemic lupus erythematosus patients. J Clin Invest. 1980 Dec;66(6):1437–1440. doi: 10.1172/JCI109999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ilyas A. A., Li S. C., Chou D. K., Li Y. T., Jungalwala F. B., Dalakas M. C., Quarles R. H. Gangliosides GM2, IV4GalNAcGM1b, and IV4GalNAcGC1a as antigens for monoclonal immunoglobulin M in neuropathy associated with gammopathy. J Biol Chem. 1988 Mar 25;263(9):4369–4373. [PubMed] [Google Scholar]
  15. Kaise S., Yasuda T., Kasukawa R., Nishimaki T., Watarai S., Tsumita T. Antiglycolipid antibodies in normal and pathologic human sera and synovial fluids. Vox Sang. 1985;49(4):292–300. doi: 10.1111/j.1423-0410.1985.tb01124.x. [DOI] [PubMed] [Google Scholar]
  16. Kasai N., Pachner A. R., Yu R. K. Anti-glycolipid antibodies and their immune complexes in multiple sclerosis. J Neurol Sci. 1986 Aug;75(1):33–42. doi: 10.1016/0022-510x(86)90048-1. [DOI] [PubMed] [Google Scholar]
  17. Kasai N., Sillerud L. O., Yu R. K. A convenient method for the preparation of asialo-GM1. Lipids. 1982 Feb;17(2):107–110. doi: 10.1007/BF02535184. [DOI] [PubMed] [Google Scholar]
  18. Kato M., Kubo S., Naiki M. Complement fixation antibodies to glycosphingolipids in sera of rare blood group p and Pk phenotypes. J Immunogenet. 1978 Feb;5(1):31–40. doi: 10.1111/j.1744-313x.1978.tb00628.x. [DOI] [PubMed] [Google Scholar]
  19. Kijimoto-Ochiai S., Takahashi W., Makita A. Anti-Forssman antibody in human sera: properties and decreased level in cancer patients. Jpn J Exp Med. 1981 Jun;51(3):149–155. [PubMed] [Google Scholar]
  20. Kitamura H., Levine P., Cheng P. J., Egeli R. A., Liu Y. P., Good R. A., Day N. K. Forssman-like antibody levels in sera of patients with lung cancer. Cancer Res. 1979 Aug;39(8):2909–2913. [PubMed] [Google Scholar]
  21. Kohn L. D., Consiglio E., DeWolf M. J., Grollman E. F., Ledley F. D., Lee G., Morris N. P. Thyrotropin receptors and gangliosides. Adv Exp Med Biol. 1980;125:487–503. doi: 10.1007/978-1-4684-7844-0_43. [DOI] [PubMed] [Google Scholar]
  22. Kohriyama T., Kusunoki S., Ariga T., Yoshino J. E., DeVries G. H., Latov N., Yu R. K. Subcellular localization of sulfated glucuronic acid-containing glycolipids reacting with anti-myelin-associated glycoprotein antibody. J Neurochem. 1987 May;48(5):1516–1522. doi: 10.1111/j.1471-4159.1987.tb05694.x. [DOI] [PubMed] [Google Scholar]
  23. Koski C. L., Chou D. K., Jungalwala F. B. Anti-peripheral nerve myelin antibodies in Guillain-Barre syndrome bind a neutral glycolipid of peripheral myelin and cross-react with Forssman antigen. J Clin Invest. 1989 Jul;84(1):280–287. doi: 10.1172/JCI114152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lacetti P., Tombaccini D., Aloj S., Grollman E. F., Kohn L. D. Gangliosides, the thyrotropin receptor, and autoimmune thyroid disease. Adv Exp Med Biol. 1984;174:355–367. doi: 10.1007/978-1-4684-1200-0_30. [DOI] [PubMed] [Google Scholar]
  25. Lee G., Grollman E. F., Aloj S. M., Kohn L. D., Winand R. J. Abnormal adenylate cyclase activity and altered membrane gangliosides in thyroid cells from patients with Graves' disease. Biochem Biophys Res Commun. 1977 Jul 11;77(1):139–146. doi: 10.1016/s0006-291x(77)80175-7. [DOI] [PubMed] [Google Scholar]
  26. Marcus D. M. A review of the immunogenic and immuno-modulatory properties of glycosphingolipids. Mol Immunol. 1984 Nov;21(11):1083–1091. doi: 10.1016/0161-5890(84)90118-4. [DOI] [PubMed] [Google Scholar]
  27. Marcus D. M. Measurement and clinical importance of antibodies to glycosphingolipids. Ann Neurol. 1990;27 (Suppl):S53–S55. doi: 10.1002/ana.410270714. [DOI] [PubMed] [Google Scholar]
  28. McGinnis S., Kohriyama T., Yu R. K., Pesce M. A., Latov N. Antibodies to sulfated glucuronic acid containing glycosphingolipids in neuropathy associated with anti-MAG antibodies and in normal subjects. J Neuroimmunol. 1988 Jan;17(2):119–126. doi: 10.1016/0165-5728(88)90019-7. [DOI] [PubMed] [Google Scholar]
  29. McKenzie J. M., Zakarija M., Sato A. Humoral immunity in Graves' disease. Clin Endocrinol Metab. 1978 Mar;7(1):31–45. doi: 10.1016/s0300-595x(78)80034-6. [DOI] [PubMed] [Google Scholar]
  30. Milgrom F., Kano K., Fjelde A., Bloom M. L. Heterophile antigens and antibodies in transplantation and tumors. Transplant Proc. 1975 Jun;7(2):201–207. [PubMed] [Google Scholar]
  31. Moore T. L., Dorner R. W. 19S IgM Forssman-type heterophile antibodies in juvenile rheumatoid arthritis. Arthritis Rheum. 1980 Nov;23(11):1262–1267. doi: 10.1002/art.1780231106. [DOI] [PubMed] [Google Scholar]
  32. Mori T., Fujii G., Kawamura A., Jr, Yasuda T., Naito Y., Tsumita T. Forssman antibody levels in sera of cancer patients. Immunol Commun. 1982;11(3):217–225. doi: 10.3109/08820138209094132. [DOI] [PubMed] [Google Scholar]
  33. Naiki M., Marcus D. M. Binding of N-acetylgalactosamine-containing compounds by a human IgM paraprotein. J Immunol. 1977 Aug;119(2):537–539. [PubMed] [Google Scholar]
  34. Nakahara K., Ohashi T., Oda T., Hirano T., Kasai M., Okumura K., Tada T. Asialo GM1 as a cell-surface marker detected in acute lymphoblastic leukemia. N Engl J Med. 1980 Mar 20;302(12):674–677. doi: 10.1056/NEJM198003203021208. [DOI] [PubMed] [Google Scholar]
  35. Nishimaki T., Kano K., Milgrom F. Studies on heterophile antibodies in rheumatoid arthritis. Arthritis Rheum. 1978 Jul-Aug;21(6):634–638. doi: 10.1002/art.1780210604. [DOI] [PubMed] [Google Scholar]
  36. Pestronk A., Adams R. N., Clawson L., Cornblath D., Kuncl R. W., Griffin D., Drachman D. B. Serum antibodies to GM1 ganglioside in amyotrophic lateral sclerosis. Neurology. 1988 Sep;38(9):1457–1461. doi: 10.1212/wnl.38.9.1457. [DOI] [PubMed] [Google Scholar]
  37. Rapport M. M., Huang Y. Present status of the immunology of gangliosides. Adv Exp Med Biol. 1984;174:15–25. doi: 10.1007/978-1-4684-1200-0_2. [DOI] [PubMed] [Google Scholar]
  38. SVENNERHOLM L. CHROMATOGRAPHIC SEPARATION OF HUMAN BRAIN GANGLIOSIDES. J Neurochem. 1963 Sep;10:613–623. doi: 10.1111/j.1471-4159.1963.tb08933.x. [DOI] [PubMed] [Google Scholar]
  39. Schwarting G. A., Kundu S. K., Marcus D. M. Reaction of antibodies that cause paroxysmal cold hemoglobinuria (PCH) with globoside and Forssman glycosphingolipids. Blood. 1979 Feb;53(2):186–192. [PubMed] [Google Scholar]
  40. Sekine M., Ariga T., Miyatake T., Kase R., Suzuki A., Yamakawa T. Gangliosides and neutral glycolipids in guinea pig adrenal glands. J Biochem. 1984 Jul;96(1):237–244. doi: 10.1093/oxfordjournals.jbchem.a134818. [DOI] [PubMed] [Google Scholar]
  41. Uemura K., Yuzawa M., Taketomi T. Immunochemical studies of lipids. V. Effect of modified hydrophobic moiety on immunogenicity and immunologic reactivity of Forssman glycolipid. Jpn J Exp Med. 1979 Feb;49(1):1–8. [PubMed] [Google Scholar]
  42. Valente W. A., Vitti P., Yavin Z., Yavin E., Rotella C. M., Grollman E. F., Toccafondi R. S., Kohn L. D. Monoclonal antibodies to the thyrotropin receptor: stimulating and blocking antibodies derived from the lymphocytes of patients with Graves disease. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6680–6684. doi: 10.1073/pnas.79.21.6680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Witkin S. S., Sonnabend J., Richards J. M., Purtilo D. T. Induction of antibody to asialo GM1 by spermatozoa and its occurrence in the sera of homosexual men with the acquired immune deficiency syndrome (AIDS). Clin Exp Immunol. 1983 Nov;54(2):346–350. [PMC free article] [PubMed] [Google Scholar]
  44. Yasuda T., Ueno J., Naito Y., Tsumita T. Antiglycolipid antibodies in human sera. Adv Exp Med Biol. 1982;152:457–465. [PubMed] [Google Scholar]
  45. Yavin E., Yavin Z., Schneider M. D., Kohn L. D. Monoclonal antibodies to the thyrotropin receptor: implications for receptor structure and the action of autoantibodies in Graves disease. Proc Natl Acad Sci U S A. 1981 May;78(5):3180–3184. doi: 10.1073/pnas.78.5.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Yoshida T., Ichikawa Y., Ito K., Homma M. Monoclonal antibodies to the thyrotropin receptor bind to a 56-kDa subunit of the thyrotropin receptor and show heterogeneous bioactivities. J Biol Chem. 1988 Nov 5;263(31):16341–16347. [PubMed] [Google Scholar]

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