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American Journal of Human Genetics logoLink to American Journal of Human Genetics
. 1989 Sep;45(3):458–464.

High-resolution analysis of a hypervariable region in the human apolipoprotein B gene.

E H Ludwig 1, W Friedl 1, B J McCarthy 1
PMCID: PMC1683401  PMID: 2773938

Abstract

A hypervariable region occurs immediately 3' of the human apolipoprotein B gene. Several allelic variants of this tandemly repeated sequence can be resolved by genomic blotting. Higher resolution among size variants may be obtained by polymerase-chain-reaction amplification of this region followed by electrophoresis in a denaturing acrylamide gel. Fourteen different alleles containing 25-52 repeats of the basic 15-bp unit were distinguished in a population study of 318 unrelated individuals. This approach should be applicable to pedigree and linkage analysis with the apolipoprotein B gene or other tandemly repeated sequence elements.

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Selected References

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