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. 1986 Apr;38(4):428–436.

Sequences homologous to the human D1S1 locus present on human chromosome 3.

B Carritt, H M Welch, N J Parry-Jones
PMCID: PMC1684798  PMID: 3010709

Abstract

We have investigated the segregation, in somatic cell hybrids, of the human D1S1 locus, previously assigned to 1p36 by in situ hybridization. We have shown that the clone which defines this locus, lambda Ch4A-H3, originates from human chromosome 3, but contains a 1.7-kilobase (kb) PstI-HindIII repetitive element that is also present on chromosome 1, probably distal to PGD. The clone recognizes restriction fragment length polymorphisms within the single-copy sequence on chromosome 3 and one for the enzyme StuI in the repeated sequence on chromosome 1. These experiments thus expose a level of complexity in the D1S1 locus not revealed by earlier in situ hybridization studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop J. O., Jones K. W. Chromosomal localization of human haemoglobin structural genes. Nature. 1972 Nov 17;240(5377):149–150. doi: 10.1038/240149a0. [DOI] [PubMed] [Google Scholar]
  2. Carritt B., Goldfarb P. S., Hooper M. L., Slack C. Chromosome assignment of a human gene for argininosuccinate synthetase expression in Chinese hamsterxhuman somatic cell hybrids. Exp Cell Res. 1977 Apr;106(1):71–78. doi: 10.1016/0014-4827(77)90242-7. [DOI] [PubMed] [Google Scholar]
  3. Carritt B., King J., Welch H. M. Gene order and localization of enzyme loci on the short arm of chromosome 1. Ann Hum Genet. 1982 Oct;46(Pt 4):329–335. doi: 10.1111/j.1469-1809.1982.tb01583.x. [DOI] [PubMed] [Google Scholar]
  4. Carritt B., Parrington J. M., Welch H. M., Povey S. Diverse origins of multiple ovarian teratomas in a single individual. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7400–7404. doi: 10.1073/pnas.79.23.7400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harper M. E., Saunders G. F. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. doi: 10.1007/BF00327364. [DOI] [PubMed] [Google Scholar]
  6. Humphries P., Barton D., McKay A. M., Humphries M. M., Carritt B. Isolation of a polymorphic DNA segment unique to human chromosome 7 by molecular cloning of hybrid cell DNA. Mol Gen Genet. 1983;190(1):143–149. doi: 10.1007/BF00330337. [DOI] [PubMed] [Google Scholar]
  7. Leder P., Tiemeier D., Enquist L. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system. Science. 1977 Apr 8;196(4286):175–177. doi: 10.1126/science.322278. [DOI] [PubMed] [Google Scholar]
  8. Lederman L. L., Kawasaki E. S., Szabo P. The rate of nucleic acid annealing to cytological preparations is increased in the presence of dextran sulfate. Anal Biochem. 1981 Oct;117(1):158–163. doi: 10.1016/0003-2697(81)90705-3. [DOI] [PubMed] [Google Scholar]
  9. Maniatis T., Hardison R. C., Lacy E., Lauer J., O'Connell C., Quon D., Sim G. K., Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. doi: 10.1016/0092-8674(78)90036-3. [DOI] [PubMed] [Google Scholar]
  10. Münke M., Lindgren V., de Martinville B., Francke U. Comparative analysis of mouse-human hybrids with rearranged chromosomes 1 by in situ hybridization and Southern blotting: high-resolution mapping of NRAS, NGFB, and AMY on human chromosome 1. Somat Cell Mol Genet. 1984 Nov;10(6):589–599. doi: 10.1007/BF01535224. [DOI] [PubMed] [Google Scholar]
  11. Skolnick M. H., Francke U. Report of the committee on human gene mapping by recombinant DNA techniques. Oslo Conference (1981): Sixth International Workshop on Human Gene Mapping. Cytogenet Cell Genet. 1982;32(1-4):194–204. doi: 10.1159/000131699. [DOI] [PubMed] [Google Scholar]
  12. Tashima M., Calabretta B., Torelli G., Scofield M., Maizel A., Saunders G. F. Presence of a highly repetitive and widely dispersed DNA sequence in the human genome. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1508–1512. doi: 10.1073/pnas.78.3.1508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]
  15. van Someren H., Beijersbergen van Henegouwen H., Los W., Wurzer-Figurelli E., Doppert B., Vervloet M., Meera Khan P. Enzyme electrophoresis on cellulose acetate gel. II. Zymogram patterns in man-Chinese hamster somatic cell hybrids. Humangenetik. 1974;25(3):189–201. doi: 10.1007/BF00281426. [DOI] [PubMed] [Google Scholar]

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