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. 1991 Mar;57(3):625–629. doi: 10.1128/aem.57.3.625-629.1991

Continuous Production of Long-Side-Chain Poly-β-Hydroxyalkanoates by Pseudomonas oleovorans

Bruce A Ramsay 1, Ilie Saracovan 1, Juliana A Ramsay 1,*, Robert H Marchessault 1
PMCID: PMC182769  PMID: 16348427

Abstract

Shake flask experiments showed that Pseudomonas oleovorans began to be growth inhibited at 4.65 g of sodium octanoate liter-1, with total inhibition at 6 g liter-1. In chemostat studies with 2 g of ammonium sulfate and 8 g of octanoate liter-1 in the feed, the maximum specific growth rate was 0.51 h-1, and the maximum specific rate of poly-β-hydroxyalkanoate (PHA) production was 0.074 g of PHA g of cellular protein-1 h-1 at a dilution rate (D) of 0.25 h-1. When the specific growth rate (μ) was <0.3 h-1, the PHA composition was relatively constant with a C4/C6/C8/C10 ratio of 0.1:1.7:20.7:1.0. At μ > 0.3 h-1, a decrease in the percentage of C8 with a concomitant increase in C10 monomers as μ increased was probably due to the effects of higher concentrations of unmetabolized octanoate in the fermentor. At D = 0.24 h-1 and an increasing carbon/nitrogen ratio, the percentage of PHA in the biomass was constant at 13% (wt/wt), indicating that nitrogen limitation did not affect PHA accumulation. Under carbon-limited conditions, the yield of biomass from substrate was 0.76 g of biomass g of octanoate-1 consumed, the yield of PHA was 0.085 g of PHA g of octanoate-1 used, and 7.9 g of octanoate was consumed for each gram of NH4+ supplied. The maintenance coefficient was 0.046 g of octanoate g of biomass-1 h-1. Replacement of sodium octanoate with octanoic acid appeared to result in transport-limited growth due to the water insolubility of the acid.

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Selected References

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  1. Anderson A. J., Haywood G. W., Dawes E. A. Biosynthesis and composition of bacterial poly(hydroxyalkanoates). Int J Biol Macromol. 1990 Apr;12(2):102–105. doi: 10.1016/0141-8130(90)90060-n. [DOI] [PubMed] [Google Scholar]
  2. Brandl H., Gross R. A., Lenz R. W., Fuller R. C. Pseudomonas oleovorans as a Source of Poly(beta-Hydroxyalkanoates) for Potential Applications as Biodegradable Polyesters. Appl Environ Microbiol. 1988 Aug;54(8):1977–1982. doi: 10.1128/aem.54.8.1977-1982.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dawes E. A., Senior P. J. The role and regulation of energy reserve polymers in micro-organisms. Adv Microb Physiol. 1973;10:135–266. doi: 10.1016/s0065-2911(08)60088-0. [DOI] [PubMed] [Google Scholar]
  4. Fritzsche K., Lenz R. W., Fuller R. C. Bacterial polyesters containing branched poly(beta-hydroxyalkanoate) units. Int J Biol Macromol. 1990 Apr;12(2):92–101. doi: 10.1016/0141-8130(90)90059-j. [DOI] [PubMed] [Google Scholar]
  5. Fritzsche K., Lenz R. W., Fuller R. C. Production of unsaturated polyesters by Pseudomonas oleovorans. Int J Biol Macromol. 1990 Apr;12(2):85–91. doi: 10.1016/0141-8130(90)90058-i. [DOI] [PubMed] [Google Scholar]
  6. Gerson D. F., Cooper D. G., Ramsay B., Zajic J. E. The effect of substrate on inhibition of Corynebacterium lepus by isonicotinic acid hydrazide (Isoniazid). Can J Microbiol. 1980 Dec;26(12):1498–1500. doi: 10.1139/m80-247. [DOI] [PubMed] [Google Scholar]
  7. Harder W., Dijkhuizen L. Physiological responses to nutrient limitation. Annu Rev Microbiol. 1983;37:1–23. doi: 10.1146/annurev.mi.37.100183.000245. [DOI] [PubMed] [Google Scholar]
  8. Huisman G. W., de Leeuw O., Eggink G., Witholt B. Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads. Appl Environ Microbiol. 1989 Aug;55(8):1949–1954. doi: 10.1128/aem.55.8.1949-1954.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lageveen R. G., Huisman G. W., Preusting H., Ketelaar P., Eggink G., Witholt B. Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates. Appl Environ Microbiol. 1988 Dec;54(12):2924–2932. doi: 10.1128/aem.54.12.2924-2932.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marchessault R. H., Monasterios C. J., Morin F. G., Sundararajan P. R. Chiral poly(beta-hydroxyalkanoates): an adaptable helix influenced by the alkane side-chain. Int J Biol Macromol. 1990 Apr;12(2):158–165. doi: 10.1016/0141-8130(90)90068-l. [DOI] [PubMed] [Google Scholar]
  11. Matin A., Veldhuis C., Stegeman V., Veenhuis M. Selective advantage of a Spirillum sp. in a carbon-limited environment. Accumulation of poly-beta-hydroxybutyric acid and its role in starvation. J Gen Microbiol. 1979 Jun;112(2):349–355. doi: 10.1099/00221287-112-2-349. [DOI] [PubMed] [Google Scholar]
  12. Oeding V., Schlegel H. G. Beta-ketothiolase from Hydrogenomonas eutropha H16 and its significance in the regulation of poly-beta-hydroxybutyrate metabolism. Biochem J. 1973 May;134(1):239–248. doi: 10.1042/bj1340239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ramsay B. A., Lomaliza K., Chavarie C., Dubé B., Bataille P., Ramsay J. A. Production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids. Appl Environ Microbiol. 1990 Jul;56(7):2093–2098. doi: 10.1128/aem.56.7.2093-2098.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ramsay B. A., Ramsay J. A., Cooper D. G. Production of Poly-beta-Hydroxyalkanoic Acid by Pseudomonas cepacia. Appl Environ Microbiol. 1989 Mar;55(3):584–589. doi: 10.1128/aem.55.3.584-589.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. de Smet M. J., Eggink G., Witholt B., Kingma J., Wynberg H. Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane. J Bacteriol. 1983 May;154(2):870–878. doi: 10.1128/jb.154.2.870-878.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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