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. 1991 Oct;57(10):2935–2939. doi: 10.1128/aem.57.10.2935-2939.1991

Genetic Transformation System for the Fungal Soybean Pathogen Cercospora kikuchii

R G Upchurch 1,*, M Ehrenshaft 1, D C Walker 1,†, L A Sanders 1,‡
PMCID: PMC183900  PMID: 16348566

Abstract

An altered β-tubulin gene that confers resistance to the fungicide benomyl was isolated from a genomic library of a UV-induced mutant of Cercospora kikuchii and used as a selectable marker for transformation. The level of benomyl resistance conferred to the transformants was at least 150-fold greater than the intrinsic resistance of the C. kikuchii recipient protoplasts. In the majority of cases, the tubulin fragment was integrated at the native β-tubulin locus, apparently by gene replacement or gene conversion. The frequency of transformation ranged from 0.2 to 6 transformants per μg of DNA, depending on the recipient strain. Transformation with linearized plasmid resulted in a higher frequency, without changing the type of integration event. Transformants were phenotypically stable after eight consecutive transfers on medium without benomyl. This is the first report of a genetic transformation system for a Cercospora species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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