Abstract
The abilities of the H+-ATPase inhibitor N, N'-dicyclohexylcarbodiimide and the antibiotic ionophore nigericin to enhance the bactericidal effect of subinhibitory concentrations of gentamicin in two strains of Staphylococcus aureus were studied. Each compound significantly increased both gentamicin uptake and killing. The use of agents which alter the plasma membrane energy state is a novel approach to enhance the activity of conventional antibiotics.
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Selected References
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- Enequist H. G., Hirst T. R., Harayama S., Hardy S. J., Randall L. L. Energy is required for maturation of exported proteins in Escherichia coli. Eur J Biochem. 1981 May 15;116(2):227–233. doi: 10.1111/j.1432-1033.1981.tb05323.x. [DOI] [PubMed] [Google Scholar]
- Mates S. M., Eisenberg E. S., Mandel L. J., Patel L., Kaback H. R., Miller M. H. Membrane potential and gentamicin uptake in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6693–6697. doi: 10.1073/pnas.79.21.6693. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mates S. M., Patel L., Kaback H. R., Miller M. H. Membrane potential in anaerobically growing Staphylococcus aureus and its relationship to gentamicin uptake. Antimicrob Agents Chemother. 1983 Apr;23(4):526–530. doi: 10.1128/aac.23.4.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMurry L. M., Cullinane J. C., Petrucci R. E., Jr, Levy S. B. Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli. Antimicrob Agents Chemother. 1981 Sep;20(3):307–313. doi: 10.1128/aac.20.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller M. H., Edberg S. C., Mandel L. J., Behar C. F., Steigbigel N. H. Gentamicin uptake in wild-type and aminoglycoside-resistant small-colony mutants of Staphylococcus aureus. Antimicrob Agents Chemother. 1980 Nov;18(5):722–729. doi: 10.1128/aac.18.5.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller M. H., Wexler M. A., Steigbigel N. H. Single and combination antibiotic therapy of Staphylococcus aureus experimental endocarditis: emergence of gentamicin-resistant mutants. Antimicrob Agents Chemother. 1978 Sep;14(3):336–343. doi: 10.1128/aac.14.3.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moellering R. C., Jr, Weinberg A. N. Studies on antibiotic syngerism against enterococci. II. Effect of various antibiotics on the uptake of 14 C-labeled streptomycin by enterococci. J Clin Invest. 1971 Dec;50(12):2580–2584. doi: 10.1172/JCI106758. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nikaido H., Nakae T. The outer membrane of Gram-negative bacteria. Adv Microb Physiol. 1979;20:163–250. doi: 10.1016/s0065-2911(08)60208-8. [DOI] [PubMed] [Google Scholar]
- Schindler H., Rosenbusch J. P. Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3751–3755. doi: 10.1073/pnas.75.8.3751. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A. The mechanism of the irreversible antimicrobial effects of penicillins: how the beta-lactam antibiotics kill and lyse bacteria. Annu Rev Microbiol. 1979;33:113–137. doi: 10.1146/annurev.mi.33.100179.000553. [DOI] [PubMed] [Google Scholar]
- Westley J. W. Polyether antibiotics: versatile carboxylic acid ionophores produced by Streptomyces. Adv Appl Microbiol. 1977;22:177–223. doi: 10.1016/s0065-2164(08)70163-1. [DOI] [PubMed] [Google Scholar]