Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1993 Jan;108(1):146–149. doi: 10.1111/j.1476-5381.1993.tb13454.x

Neurokinin-induced changes in pial artery diameter in the anaesthetized guinea-pig.

D T Beattie 1, C M Stubbs 1, H E Connor 1, W Feniuk 1
PMCID: PMC1907708  PMID: 7679026

Abstract

1. The effects of selective neurokinin agents on pial artery diameter, measured with an on-line image analyser, have been studied in anaesthetized guinea-pigs in order to characterize the neurokinin receptors present on pial arteries. 2. Perivascular injection of either substance P (0.01-1 microM) or the selective NK1 receptor agonists, substance P methyl ester (SPOMe, 0.01-1 microM) and GR73632 (0.1 microM), increased pial artery diameter. 3. In contrast, the selective NK2 receptor agonist, GR64349 (1 microM), produced a small vasoconstriction while the NK3 receptor-selective agonist, senktide (1 microM) was inactive. 4. Co-administration of GR82334 (1 microM), a selective NK1 receptor antagonist, inhibited the vasodilatation produced by SPOMe (0.1 microM) but not that caused by calcitonin gene-related peptide (CGRP, 0.01 microM). 5. The results are consistent with an involvement of NK1 receptors in the neurokinin-induced increase in guinea-pig pial artery diameter.

Full text

PDF
148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brain S. D., Williams T. J., Tippins J. R., Morris H. R., MacIntyre I. Calcitonin gene-related peptide is a potent vasodilator. Nature. 1985 Jan 3;313(5997):54–56. doi: 10.1038/313054a0. [DOI] [PubMed] [Google Scholar]
  2. D'Orléans-Juste P., Dion S., Drapeau G., Regoli D. Different receptors are involved in the endothelium-mediated relaxation and the smooth muscle contraction of the rabbit pulmonary artery in response to substance P and related neurokinins. Eur J Pharmacol. 1986 Jun 5;125(1):37–44. doi: 10.1016/0014-2999(86)90081-6. [DOI] [PubMed] [Google Scholar]
  3. Edvinsson L., McCulloch J., Uddman R. Substance P: immunohistochemical localization and effect upon cat pial arteries in vitro and in situ. J Physiol. 1981 Sep;318:251–258. doi: 10.1113/jphysiol.1981.sp013862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edvinsson L., Rosendal-Helgesen S., Uddman R. Substance P: localization, concentration and release in cerebral arteries, choroid plexus and dura mater. Cell Tissue Res. 1983;234(1):1–7. doi: 10.1007/BF00217397. [DOI] [PubMed] [Google Scholar]
  5. Goadsby P. J., Edvinsson L., Ekman R. Release of vasoactive peptides in the extracerebral circulation of humans and the cat during activation of the trigeminovascular system. Ann Neurol. 1988 Feb;23(2):193–196. doi: 10.1002/ana.410230214. [DOI] [PubMed] [Google Scholar]
  6. Hagan R. M., Ireland S. J., Jordan C. C., Beresford I. J., Deal M. J., Ward P. Receptor-selective, peptidase-resistant agonists at neurokinin NK-1 and NK-2 receptors: new tools for investigating neurokinin function. Neuropeptides. 1991 Jun;19(2):127–135. doi: 10.1016/0143-4179(91)90142-6. [DOI] [PubMed] [Google Scholar]
  7. Humphrey P. P., Feniuk W. Mode of action of the anti-migraine drug sumatriptan. Trends Pharmacol Sci. 1991 Dec;12(12):444–446. doi: 10.1016/0165-6147(91)90630-b. [DOI] [PubMed] [Google Scholar]
  8. Ireland S. J., Bailey F., Cook A., Hagan R. M., Jordan C. C., Stephens-Smith M. L. Receptors mediating tachykinin-induced contractile responses in guinea-pig trachea. Br J Pharmacol. 1991 Jun;103(2):1463–1469. doi: 10.1111/j.1476-5381.1991.tb09812.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jansen I., Alafaci C., McCulloch J., Uddman R., Edvinsson L. Tachykinins (substance P, neurokinin A, neuropeptide K, and neurokinin B) in the cerebral circulation: vasomotor responses in vitro and in situ. J Cereb Blood Flow Metab. 1991 Jul;11(4):567–575. doi: 10.1038/jcbfm.1991.105. [DOI] [PubMed] [Google Scholar]
  10. Laufer R., Gilon C., Chorev M., Selinger Z. Characterization of a neurokinin B receptor site in rat brain using a highly selective radioligand. J Biol Chem. 1986 Aug 5;261(22):10257–10263. [PubMed] [Google Scholar]
  11. Lee C. M., Campbell N. J., Williams B. J., Iversen L. L. Multiple tachykinin binding sites in peripheral tissues and in brain. Eur J Pharmacol. 1986 Nov 4;130(3):209–217. doi: 10.1016/0014-2999(86)90270-0. [DOI] [PubMed] [Google Scholar]
  12. Liu-Chen L. Y., Mayberg M. R., Moskowitz M. A. Immunohistochemical evidence for a substance P-containing trigeminovascular pathway to pial arteries in cats. Brain Res. 1983 May 23;268(1):162–166. doi: 10.1016/0006-8993(83)90402-x. [DOI] [PubMed] [Google Scholar]
  13. Markowitz S., Saito K., Moskowitz M. A. Neurogenically mediated leakage of plasma protein occurs from blood vessels in dura mater but not brain. J Neurosci. 1987 Dec;7(12):4129–4136. doi: 10.1523/JNEUROSCI.07-12-04129.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCulloch J., Uddman R., Kingman T. A., Edvinsson L. Calcitonin gene-related peptide: functional role in cerebrovascular regulation. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5731–5735. doi: 10.1073/pnas.83.15.5731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Moskowitz M. A., Brody M., Liu-Chen L. Y. In vitro release of immunoreactive substance P from putative afferent nerve endings in bovine pia arachnoid. Neuroscience. 1983 Aug;9(4):809–814. doi: 10.1016/0306-4522(83)90269-5. [DOI] [PubMed] [Google Scholar]
  16. Moskowitz M. A., Buzzi M. G., Sakas D. E., Linnik M. D. Pain mechanisms underlying vascular headaches. Progress Report 1989. Rev Neurol (Paris) 1989;145(3):181–193. [PubMed] [Google Scholar]
  17. Moskowitz M. A. The neurobiology of vascular head pain. Ann Neurol. 1984 Aug;16(2):157–168. doi: 10.1002/ana.410160202. [DOI] [PubMed] [Google Scholar]
  18. Regoli D., Drapeau G., Dion S., D'Orléans-Juste P. Receptors for substance P and related neurokinins. Pharmacology. 1989;38(1):1–15. doi: 10.1159/000138512. [DOI] [PubMed] [Google Scholar]
  19. Skofitsch G., Jacobowitz D. M. Calcitonin gene-related peptide coexists with substance P in capsaicin sensitive neurons and sensory ganglia of the rat. Peptides. 1985 Jul-Aug;6(4):747–754. doi: 10.1016/0196-9781(85)90179-2. [DOI] [PubMed] [Google Scholar]
  20. Stubbs C. M., Waldron G. J., Connor H. E., Feniuk W. Characterization of the receptor mediating relaxation to substance P in canine middle cerebral artery: no evidence for involvement of substance P in neurogenically mediated relaxation. Br J Pharmacol. 1992 Apr;105(4):875–880. doi: 10.1111/j.1476-5381.1992.tb09071.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wahl M., Schilling L., Whalley E. T. Cerebrovascular effects of prostanoids. In-situ studies in pial arteries of the cat. Naunyn Schmiedebergs Arch Pharmacol. 1989 Sep;340(3):314–320. doi: 10.1007/BF00168516. [DOI] [PubMed] [Google Scholar]
  22. Watson S. P., Sandberg B. E., Hanley M. R., Iversen L. L. Tissue selectivity of substance P alkyl esters: suggesting multiple receptors. Eur J Pharmacol. 1983 Jan 28;87(1):77–84. doi: 10.1016/0014-2999(83)90052-3. [DOI] [PubMed] [Google Scholar]
  23. Wormser U., Laufer R., Hart Y., Chorev M., Gilon C., Selinger Z. Highly selective agonists for substance P receptor subtypes. EMBO J. 1986 Nov;5(11):2805–2808. doi: 10.1002/j.1460-2075.1986.tb04571.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES