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. 1996 Oct;70(10):7295–7300. doi: 10.1128/jvi.70.10.7295-7300.1996

Nonrandom segregation of parental alleles in reovirus reassortants.

M L Nibert 1, R L Margraf 1, K M Coombs 1
PMCID: PMC190792  PMID: 8794386

Abstract

To test for nonrandom segregations among their 10 genomic RNA segments, we examined a set of 83 reassortants derived from mammalian reovirus type 1 Lang and type 3 Dearing. After confirming the genotypes of the reassortants, we performed statistical analyses on the distributions of parental alleles for each of the 10 gene segments, as well as for the 45 possible pairings of the 10 segments. The analyses revealed nonrandom associations of parental alleles in the L1-L2, L1-M1, L1-S1, and L3-S1 segment pairs, at levels indicating high statistical significance (P < 0.005). Such associations may reflect specific interactions between viral components (protein-protein, protein-RNA, or RNA-RNA) and may influence both the evolution of reoviruses in nature and their genetic analysis in the laboratory. The data may also support an hypothesis that reovirus reassortants commonly contain mutations that improve their fitness for independent replication.

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Selected References

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  1. Cashdollar L. W. Characterization and structural localization of the reovirus lambda 3 protein. Res Virol. 1994 Sep-Oct;145(5):277–285. doi: 10.1016/s0923-2516(07)80032-x. [DOI] [PubMed] [Google Scholar]
  2. Cleveland D. R., Zarbl H., Millward S. Reovirus guanylyltransferase is L2 gene product lambda 2. J Virol. 1986 Oct;60(1):307–311. doi: 10.1128/jvi.60.1.307-311.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coombs K. M., Fields B. N., Harrison S. C. Crystallization of the reovirus type 3 Dearing core. Crystal packing is determined by the lambda 2 protein. J Mol Biol. 1990 Sep 5;215(1):1–5. doi: 10.1016/s0022-2836(05)80089-0. [DOI] [PubMed] [Google Scholar]
  4. Cross R. K., Fields B. N. Use of an aberrant polypeptide as a marker in three-factor crosses: further evidence for independent reassortment as the mechanism of recombination between temperature-sensitive mutants of reovirus type 3. Virology. 1976 Oct 15;74(2):345–362. doi: 10.1016/0042-6822(76)90341-x. [DOI] [PubMed] [Google Scholar]
  5. Drayna D., Fields B. N. Activation and characterization of the reovirus transcriptase: genetic analysis. J Virol. 1982 Jan;41(1):110–118. doi: 10.1128/jvi.41.1.110-118.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dryden K. A., Wang G., Yeager M., Nibert M. L., Coombs K. M., Furlong D. B., Fields B. N., Baker T. S. Early steps in reovirus infection are associated with dramatic changes in supramolecular structure and protein conformation: analysis of virions and subviral particles by cryoelectron microscopy and image reconstruction. J Cell Biol. 1993 Sep;122(5):1023–1041. doi: 10.1083/jcb.122.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fields B. N. Temperature-sensitive mutants of reovirus type 3 features of genetic recombination. Virology. 1971 Oct;46(1):142–148. doi: 10.1016/0042-6822(71)90013-4. [DOI] [PubMed] [Google Scholar]
  8. Gombold J. L., Ramig R. F. Analysis of reassortment of genome segments in mice mixedly infected with rotaviruses SA11 and RRV. J Virol. 1986 Jan;57(1):110–116. doi: 10.1128/jvi.57.1.110-116.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goral M. I., Mochow-Grundy M., Dermody T. S. Sequence diversity within the reovirus S3 gene: reoviruses evolve independently of host species, geographic locale, and date of isolation. Virology. 1996 Feb 1;216(1):265–271. doi: 10.1006/viro.1996.0059. [DOI] [PubMed] [Google Scholar]
  10. Graham A., Kudesia G., Allen A. M., Desselberger U. Reassortment of human rotavirus possessing genome rearrangements with bovine rotavirus: evidence for host cell selection. J Gen Virol. 1987 Jan;68(Pt 1):115–122. doi: 10.1099/0022-1317-68-1-115. [DOI] [PubMed] [Google Scholar]
  11. Haller B. L., Barkon M. L., Vogler G. P., Virgin H. W., 4th Genetic mapping of reovirus virulence and organ tropism in severe combined immunodeficient mice: organ-specific virulence genes. J Virol. 1995 Jan;69(1):357–364. doi: 10.1128/jvi.69.1.357-364.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Joklik W. K., Roner M. R. What reassorts when reovirus genome segments reassort? J Biol Chem. 1995 Mar 3;270(9):4181–4184. doi: 10.1074/jbc.270.9.4181. [DOI] [PubMed] [Google Scholar]
  13. Kavenoff R., Talcove D., Mudd J. A. Genome-sized RNA from reovirus particles. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4317–4321. doi: 10.1073/pnas.72.11.4317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kedl R., Schmechel S., Schiff L. Comparative sequence analysis of the reovirus S4 genes from 13 serotype 1 and serotype 3 field isolates. J Virol. 1995 Jan;69(1):552–559. doi: 10.1128/jvi.69.1.552-559.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lubeck M. D., Palese P., Schulman J. L. Nonrandom association of parental genes in influenza A virus recombinants. Virology. 1979 May;95(1):269–274. doi: 10.1016/0042-6822(79)90430-6. [DOI] [PubMed] [Google Scholar]
  16. Mustoe T. A., Ramig R. F., Sharpe A. H., Fields B. N. Genetics of reovirus: identification of the ds RNA segments encoding the polypeptides of the mu and sigma size classes. Virology. 1978 Sep;89(2):594–604. doi: 10.1016/0042-6822(78)90200-3. [DOI] [PubMed] [Google Scholar]
  17. Nibert M. L., Furlong D. B., Fields B. N. Mechanisms of viral pathogenesis. Distinct forms of reoviruses and their roles during replication in cells and host. J Clin Invest. 1991 Sep;88(3):727–734. doi: 10.1172/JCI115369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ramig R. F., Mustoe T. A., Sharpe A. H., Fields B. N. A genetic map of reovirus. II. Assignment of the double-stranded RNA-negative mutant groups C, D, and E to genome segments. Virology. 1978 Apr;85(2):531–534. doi: 10.1016/0042-6822(78)90459-2. [DOI] [PubMed] [Google Scholar]
  19. Ramig R. F., Ward R. L. Genomic segment reassortment in rotaviruses and other reoviridae. Adv Virus Res. 1991;39:163–207. doi: 10.1016/s0065-3527(08)60795-2. [DOI] [PubMed] [Google Scholar]
  20. Roner M. R., Lin P. N., Nepluev I., Kong L. J., Joklik W. K. Identification of signals required for the insertion of heterologous genome segments into the reovirus genome. Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12362–12366. doi: 10.1073/pnas.92.26.12362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Roner M. R., Sutphin L. A., Joklik W. K. Reovirus RNA is infectious. Virology. 1990 Dec;179(2):845–852. doi: 10.1016/0042-6822(90)90153-i. [DOI] [PubMed] [Google Scholar]
  22. Sharpe A. H., Fields B. N. Reovirus inhibition of cellular DNA synthesis: role of the S1 gene. J Virol. 1981 Apr;38(1):389–392. doi: 10.1128/jvi.38.1.389-392.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sherry B., Blum M. A. Multiple viral core proteins are determinants of reovirus-induced acute myocarditis. J Virol. 1994 Dec;68(12):8461–8465. doi: 10.1128/jvi.68.12.8461-8465.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Urquidi V., Bishop D. H. Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses. J Gen Virol. 1992 Sep;73(Pt 9):2255–2265. doi: 10.1099/0022-1317-73-9-2255. [DOI] [PubMed] [Google Scholar]
  25. Vasquez C., Kleinschmidt A. K. Electron microscopy of RNA strands released from individual Reovirus particles. J Mol Biol. 1968 May 28;34(1):137–147. doi: 10.1016/0022-2836(68)90240-4. [DOI] [PubMed] [Google Scholar]
  26. Virgin H. W., 4th, Mann M. A., Fields B. N., Tyler K. L. Monoclonal antibodies to reovirus reveal structure/function relationships between capsid proteins and genetics of susceptibility to antibody action. J Virol. 1991 Dec;65(12):6772–6781. doi: 10.1128/jvi.65.12.6772-6781.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yin P., Cheang M., Coombs K. M. The M1 gene is associated with differences in the temperature optimum of the transcriptase activity in reovirus core particles. J Virol. 1996 Feb;70(2):1223–1227. doi: 10.1128/jvi.70.2.1223-1227.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]

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