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. 1991 Feb;102(2):396–400. doi: 10.1111/j.1476-5381.1991.tb12185.x

Adenosine- and alpha,beta-methylene ATP-induced differential inhibition of cholinergic and non-cholinergic neurogenic responses in rat urinary bladder.

S C Parija 1, V Raviprakash 1, S K Mishra 1
PMCID: PMC1918022  PMID: 1673071

Abstract

1. The effects of adenosine and alpha,beta-methylene adenosine triphosphate (alpha,beta-Me ATP) on single pulse-induced neurogenic responses and contractions caused by exogenously applied acetylcholine (ACh) and adenosine triphosphate (ATP) were examined in rat urinary bladder. 2. Application of single pulse stimulation (1 ms; 80 V) evoked a biphasic contractile response (abolished by tetrodotoxin, 0.5 x 10(-7) M) consisting of a fast (time to peak: 1.02 +/- 0.07 s) and a slow component (time to peak: 4.92 +/- 1.6 s). The selective inhibition of the slow component by atropine (3 x 10(-6) M) suggests the participation of both cholinergic and non-cholinergic neurotransmitters. 3. alpha,beta-Me ATP (5 x 10(-6) M) abolished ATP (10(-4) M)-induced contractions without altering those to ACh (10(-6) M). Further, the selective inhibition of the fast component of the neurogenic response by alpha,beta-Me ATP is suggestive of the contribution of endogenous ATP to the non-cholinergic component. 4. Adenosine (10(-8) M to 10(-4) M) caused dose-related differential inhibition of the fast (IC50, 1.04 +/- 0.25 x 10(-5) M) and slow (IC50, 2.18 +/- 0.69 x 10(-6) M) components, thereby further supporting two modes of neurotransmission in bladder. 5. Theophylline (10(-4) M) antagonized the inhibitory effects of adenosine on the non-cholinergic component, thereby implicating the participation of P1-purinoceptors in neuromodulation. In contrast, theophylline at this concentration enhanced the adenosine-induced inhibition of the cholinergic component. component.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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