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. 1994 Aug;176(15):4754–4756. doi: 10.1128/jb.176.15.4754-4756.1994

Halobacterial S9 operon contains two genes encoding proteins homologous to subunits shared by eukaryotic RNA polymerases I, II, and III.

K McKune 1, N A Woychik 1
PMCID: PMC196299  PMID: 8045907

Abstract

One key component of the eukaryotic transcriptional apparatus is the multisubunit enzyme RNA polymerase II. We have discovered that two of the subunits shared by the three nuclear RNA polymerases in the yeast Saccharomyces cerevisiae, RPB6 and RPB10, have counterparts among the Archaea.

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Selected References

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  1. Allison L. A., Moyle M., Shales M., Ingles C. J. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases. Cell. 1985 Sep;42(2):599–610. doi: 10.1016/0092-8674(85)90117-5. [DOI] [PubMed] [Google Scholar]
  2. Amegadzie B. Y., Ahn B. Y., Moss B. Characterization of a 7-kilodalton subunit of vaccinia virus DNA-dependent RNA polymerase with structural similarities to the smallest subunit of eukaryotic RNA polymerase II. J Virol. 1992 May;66(5):3003–3010. doi: 10.1128/jvi.66.5.3003-3010.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boeke J. D., Trueheart J., Natsoulis G., Fink G. R. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 1987;154:164–175. doi: 10.1016/0076-6879(87)54076-9. [DOI] [PubMed] [Google Scholar]
  4. Carles C., Treich I., Bouet F., Riva M., Sentenac A. Two additional common subunits, ABC10 alpha and ABC10 beta, are shared by yeast RNA polymerases. J Biol Chem. 1991 Dec 15;266(35):24092–24096. [PubMed] [Google Scholar]
  5. Dayhoff M. O., Barker W. C., Hunt L. T. Establishing homologies in protein sequences. Methods Enzymol. 1983;91:524–545. doi: 10.1016/s0076-6879(83)91049-2. [DOI] [PubMed] [Google Scholar]
  6. Dillon P. J., Rosen C. A. A rapid method for the construction of synthetic genes using the polymerase chain reaction. Biotechniques. 1990 Sep;9(3):298–300. [PubMed] [Google Scholar]
  7. Jaehning J. A. Sigma factor relatives in eukaryotes. Science. 1991 Aug 23;253(5022):859–859. doi: 10.1126/science.1876846. [DOI] [PubMed] [Google Scholar]
  8. Klenk H. P., Palm P., Lottspeich F., Zillig W. Component H of the DNA-dependent RNA polymerases of Archaea is homologous to a subunit shared by the three eucaryal nuclear RNA polymerases. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):407–410. doi: 10.1073/pnas.89.1.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kolodziej P., Young R. A. RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus. Mol Cell Biol. 1989 Dec;9(12):5387–5394. doi: 10.1128/mcb.9.12.5387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Krömer W. J., Arndt E. Halobacterial S9 operon. Three ribosomal protein genes are cotranscribed with genes encoding a tRNA(Leu), the enolase, and a putative membrane protein in the archaebacterium Haloarcula (Halobacterium) marismortui. J Biol Chem. 1991 Dec 25;266(36):24573–24579. [PubMed] [Google Scholar]
  11. Leffers H., Gropp F., Lottspeich F., Zillig W., Garrett R. A. Sequence, organization, transcription and evolution of RNA polymerase subunit genes from the archaebacterial extreme halophiles Halobacterium halobium and Halococcus morrhuae. J Mol Biol. 1989 Mar 5;206(1):1–17. doi: 10.1016/0022-2836(89)90519-6. [DOI] [PubMed] [Google Scholar]
  12. McKune K., Richards K. L., Edwards A. M., Young R. A., Woychik N. A. RPB7, one of two dissociable subunits of yeast RNA polymerase II, is essential for cell viability. Yeast. 1993 Mar;9(3):295–299. doi: 10.1002/yea.320090309. [DOI] [PubMed] [Google Scholar]
  13. McKune K., Woychik N. A. Functional substitution of an essential yeast RNA polymerase subunit by a highly conserved mammalian counterpart. Mol Cell Biol. 1994 Jun;14(6):4155–4159. doi: 10.1128/mcb.14.6.4155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pühler G., Leffers H., Gropp F., Palm P., Klenk H. P., Lottspeich F., Garrett R. A., Zillig W. Archaebacterial DNA-dependent RNA polymerases testify to the evolution of the eukaryotic nuclear genome. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4569–4573. doi: 10.1073/pnas.86.12.4569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scholzen T., Arndt E. The alpha-operon equivalent genome region in the extreme halophilic archaebacterium Haloarcula (Halobacterium) marismortui. J Biol Chem. 1992 Jun 15;267(17):12123–12130. [PubMed] [Google Scholar]
  17. Sweetser D., Nonet M., Young R. A. Prokaryotic and eukaryotic RNA polymerases have homologous core subunits. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1192–1196. doi: 10.1073/pnas.84.5.1192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Treich I., Carles C., Riva M., Sentenac A. RPC10 encodes a new mini subunit shared by yeast nuclear RNA polymerases. Gene Expr. 1992;2(1):31–37. [PMC free article] [PubMed] [Google Scholar]
  19. Woychik N. A., Lane W. S., Young R. A. Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes. J Biol Chem. 1991 Oct 5;266(28):19053–19055. [PubMed] [Google Scholar]
  20. Woychik N. A., Liao S. M., Kolodziej P. A., Young R. A. Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev. 1990 Mar;4(3):313–323. doi: 10.1101/gad.4.3.313. [DOI] [PubMed] [Google Scholar]
  21. Woychik N. A., McKune K., Lane W. S., Young R. A. Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III. Gene Expr. 1993;3(1):77–82. [PMC free article] [PubMed] [Google Scholar]
  22. Woychik N. A., Young R. A. RNA polymerase II subunit RPB10 is essential for yeast cell viability. J Biol Chem. 1990 Oct 15;265(29):17816–17819. [PubMed] [Google Scholar]
  23. Woychik N. A., Young R. A. RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth. Mol Cell Biol. 1989 Jul;9(7):2854–2859. doi: 10.1128/mcb.9.7.2854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Young R. A. RNA polymerase II. Annu Rev Biochem. 1991;60:689–715. doi: 10.1146/annurev.bi.60.070191.003353. [DOI] [PubMed] [Google Scholar]

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