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. 1994 Nov;176(22):7087–7090. doi: 10.1128/jb.176.22.7087-7090.1994

A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration.

J W Vrijbloed 1, J Madoń 1, L Dijkhuizen 1
PMCID: PMC197085  PMID: 7961475

Abstract

Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.

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Selected References

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