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. 1992 Jun;65(6):798–802. doi: 10.1038/bjc.1992.171

A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.

S N Powell 1, S J Whitaker 1, S M Edwards 1, T J McMillan 1
PMCID: PMC1977752  PMID: 1616851

Abstract

DNA repair was measured in an ionising radiation-sensitive mutant of a human bladder carcinoma cell line. No difference in the rate or extent of double-strand break rejoining was found using the techniques of neutral filter elution and pulsed-field gel electrophoresis. In contrast, significant differences in repair fidelity, measured by plasmid reconstitution, were found. The parent line had a repair fidelity of 84.7% compared with 58.9% for S40b (P = 0.0003). It is suggested that repair fidelity can be an important determinant of radiosensitivity in human tumour cells.

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Selected References

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