Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1992 Jun;65(6):798–802. doi: 10.1038/bjc.1992.171

A DNA repair defect in a radiation-sensitive clone of a human bladder carcinoma cell line.

S N Powell 1, S J Whitaker 1, S M Edwards 1, T J McMillan 1
PMCID: PMC1977752  PMID: 1616851

Abstract

DNA repair was measured in an ionising radiation-sensitive mutant of a human bladder carcinoma cell line. No difference in the rate or extent of double-strand break rejoining was found using the techniques of neutral filter elution and pulsed-field gel electrophoresis. In contrast, significant differences in repair fidelity, measured by plasmid reconstitution, were found. The parent line had a repair fidelity of 84.7% compared with 58.9% for S40b (P = 0.0003). It is suggested that repair fidelity can be an important determinant of radiosensitivity in human tumour cells.

Full text

PDF
801

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes G., Rine J. Regulated expression of endonuclease EcoRI in Saccharomyces cerevisiae: nuclear entry and biological consequences. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1354–1358. doi: 10.1073/pnas.82.5.1354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blöcher D., Einspenner M., Zajackowski J. CHEF electrophoresis, a sensitive technique for the determination of DNA double-strand breaks. Int J Radiat Biol. 1989 Oct;56(4):437–448. doi: 10.1080/09553008914551591. [DOI] [PubMed] [Google Scholar]
  3. Bradley M. O., Kohn K. W. X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acids Res. 1979 Oct 10;7(3):793–804. doi: 10.1093/nar/7.3.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bryant P. E., Birch D. A., Jeggo P. A. High chromosomal sensitivity of Chinese hamster xrs 5 cells to restriction endonuclease induced DNA double-strand breaks. Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Oct;52(4):537–554. doi: 10.1080/09553008714552041. [DOI] [PubMed] [Google Scholar]
  5. Bryant P. E., Blöcher D. Measurement of the kinetics of DNA double strand break repair in Ehrlich ascites tumour cells using the unwinding method. Int J Radiat Biol Relat Stud Phys Chem Med. 1980 Sep;38(3):335–347. doi: 10.1080/09553008014551691. [DOI] [PubMed] [Google Scholar]
  6. Bryant P. E. Use of restriction endonucleases to study relationships between DNA double-strand breaks, chromosomal aberrations and other end-points in mammalian cells. Int J Radiat Biol. 1988 Dec;54(6):869–890. doi: 10.1080/09553008814552291. [DOI] [PubMed] [Google Scholar]
  7. Coquerelle T. M., Weibezahn K. F., Lücke-Huhle C. Rejoining of double strand breaks in normal human and ataxia-telangiectasia fibroblasts after exposure to 60Co gamma-rays, 241Am alpha-particles or bleomycin. Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Feb;51(2):209–218. doi: 10.1080/09553008714550711. [DOI] [PubMed] [Google Scholar]
  8. Cox R., Debenham P. G., Masson W. K., Webb M. B. Ataxia-telangiectasia: a human mutation giving high-frequency misrepair of DNA double-stranded scissions. Mol Biol Med. 1986 Jun;3(3):229–244. [PubMed] [Google Scholar]
  9. Debenham P. G., Jones N. J., Webb M. B. Vector-mediated DNA double-strand break repair analysis in normal, and radiation-sensitive, Chinese hamster V79 cells. Mutat Res. 1988 May;199(1):1–9. doi: 10.1016/0027-5107(88)90224-2. [DOI] [PubMed] [Google Scholar]
  10. Debenham P. G., Webb M. B., Stretch A., Thacker J. Examination of vectors with two dominant, selectable genes for DNA repair and mutation studies in mammalian cells. Mutat Res. 1988 May;199(1):145–158. doi: 10.1016/0027-5107(88)90240-0. [DOI] [PubMed] [Google Scholar]
  11. Evans H. H., Ricanati M., Horng M. F. Deficiency in DNA repair in mouse lymphoma strain L5178Y-S. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7562–7566. doi: 10.1073/pnas.84.21.7562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Folger K. R., Wong E. A., Wahl G., Capecchi M. R. Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules. Mol Cell Biol. 1982 Nov;2(11):1372–1387. doi: 10.1128/mcb.2.11.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Giaccia A., Weinstein R., Hu J., Stamato T. D. Cell cycle-dependent repair of double-strand DNA breaks in a gamma-ray-sensitive Chinese hamster cell. Somat Cell Mol Genet. 1985 Sep;11(5):485–491. doi: 10.1007/BF01534842. [DOI] [PubMed] [Google Scholar]
  14. Jeggo P. A., Kemp L. M. X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents. Mutat Res. 1983 Dec;112(6):313–327. doi: 10.1016/0167-8817(83)90026-3. [DOI] [PubMed] [Google Scholar]
  15. Jeggo P. A. Studies on mammalian mutants defective in rejoining double-strand breaks in DNA. Mutat Res. 1990 Jul;239(1):1–16. doi: 10.1016/0165-1110(90)90028-a. [DOI] [PubMed] [Google Scholar]
  16. Jones N. J., Cox R., Thacker J. Six complementation groups for ionising-radiation sensitivity in Chinese hamster cells. Mutat Res. 1988 Mar;193(2):139–144. doi: 10.1016/0167-8817(88)90044-2. [DOI] [PubMed] [Google Scholar]
  17. Jones N. J., Stewart S. A., Thompson L. H. Biochemical and genetic analysis of the Chinese hamster mutants irs1 and irs2 and their comparison to cultured ataxia telangiectasia cells. Mutagenesis. 1990 Jan;5(1):15–23. doi: 10.1093/mutage/5.1.15. [DOI] [PubMed] [Google Scholar]
  18. Kemp L. M., Sedgwick S. G., Jeggo P. A. X-ray sensitive mutants of Chinese hamster ovary cells defective in double-strand break rejoining. Mutat Res. 1984 Nov-Dec;132(5-6):189–196. doi: 10.1016/0167-8817(84)90037-3. [DOI] [PubMed] [Google Scholar]
  19. McMillan T. J., Cassoni A. M., Edwards S., Holmes A., Peacock J. H. The relationship of DNA double-strand break induction to radiosensitivity in human tumour cell lines. Int J Radiat Biol. 1990 Sep;58(3):427–438. doi: 10.1080/09553009014551781. [DOI] [PubMed] [Google Scholar]
  20. McMillan T. J., Holmes A. The isolation and partial characterization of a radiation-sensitive clone of a human bladder carcinoma cell line. Radiat Res. 1991 Dec;128(3):301–305. [PubMed] [Google Scholar]
  21. Mulligan R. C., Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072–2076. doi: 10.1073/pnas.78.4.2072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Obe G., Von der Hude W., Scheutwinkel-Reich M., Basler A. The restriction endonuclease Alu I induces chromosomal aberrations and mutations in the hypoxanthine phosphoribosyltransferase locus, but not in the Na+/K+ ATPase locus in V79 hamster cells. Mutat Res. 1986 May;174(1):71–74. doi: 10.1016/0165-7992(86)90079-5. [DOI] [PubMed] [Google Scholar]
  23. Painter R. B. Radioresistant DNA synthesis: an intrinsic feature of ataxia telangiectasia. Mutat Res. 1981 Nov;84(1):183–190. doi: 10.1016/0027-5107(81)90061-0. [DOI] [PubMed] [Google Scholar]
  24. Powell S., McMillan T. J. Clonal variation of DNA repair in a human glioma cell line. Radiother Oncol. 1991 Aug;21(4):225–232. doi: 10.1016/0167-8140(91)90046-j. [DOI] [PubMed] [Google Scholar]
  25. Sato K., Hieda N. Isolation and characterization of a mutant mouse lymphoma cell sensitive to methyl methanesulfonate and X rays. Radiat Res. 1979 Apr;78(1):167–171. [PubMed] [Google Scholar]
  26. Schwartz J. L., Rotmensch J., Giovanazzi S., Cohen M. B., Weichselbaum R. R. Faster repair of DNA double-strand breaks in radioresistant human tumor cells. Int J Radiat Oncol Biol Phys. 1988 Oct;15(4):907–912. doi: 10.1016/0360-3016(88)90125-3. [DOI] [PubMed] [Google Scholar]
  27. Stamato T. D., Denko N. Asymmetric field inversion gel electrophoresis: a new method for detecting DNA double-strand breaks in mammalian cells. Radiat Res. 1990 Feb;121(2):196–205. [PubMed] [Google Scholar]
  28. Whitaker S. J., McMillan T. J. Oxygen effect for DNA double-strand break induction determined by pulsed-field gel electrophoresis. Int J Radiat Biol. 1992 Jan;61(1):29–41. doi: 10.1080/09553009214550591. [DOI] [PubMed] [Google Scholar]
  29. Wlodek D., Hittelman W. N. The repair of double-strand DNA breaks correlates with radiosensitivity of L5178Y-S and L5178Y-R cells. Radiat Res. 1987 Oct;112(1):146–155. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES