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. 1994 Jun;60(6):1921–1926. doi: 10.1128/aem.60.6.1921-1926.1994

In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

J L Romalde 1, M K Estes 1, G Szücs 1, R L Atmar 1, C M Woodley 1, T G Metcalf 1
PMCID: PMC201581  PMID: 8031087

Abstract

An in situ transcription method was developed to detect hepatitis A virus RNA in both cell cultures and shellfish tissues. Radiolabeled cDNA copies were synthesized in situ by reverse transcriptase-directed transcription after annealing with a specific primer to the viral RNA. Both tritium (3H) and 35S were useful in the in situ transcription reaction, but the use of 3H resulted in a lower background and finer detail in the localization of viral particles. Application of the method to different organs of oysters which had bioaccumulated hepatitis A virus allowed the first in situ localization of the virus, specifically in stomach and hepatopancreatic tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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