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. 1994 Jul;60(7):2330–2338. doi: 10.1128/aem.60.7.2330-2338.1994

Regulation of chloro- and methylphenol degradation in Comamonas testosteroni JH5.

J Hollender 1, W Dott 1, J Hopp 1
PMCID: PMC201651  PMID: 8074514

Abstract

Comamonas testosteroni JH5 was isolated from a mixed bacterial culture enriched on different chloro- and methylphenols. The strain completely mineralized a mixture consisting of 4-chlorophenol (4-CP) and 4-methylphenol (4-MP). During degradation of the mixture, 4-hydroxybenzyl alcohol, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, and 4-chlorocatechol were detected as short-lived intermediates. Mineralization of 4-CP and that of 4-MP occurred successively and were accompanied by diauxic growth, whereas 4-CP and 2-methylphenol were mineralized simultaneously. It was ascertained that neither a reversible enzyme inhibition nor potential toxic intermediates caused the observed diauxie. Some facts support the hypothesis that the successive degradation of 4-CP and 4-MP is regulated on the level of transcription. C. testosteroni JH5 contained a meta-cleaving enzyme when pregrown on 4-CP and the isomeric monomethylphenols. Inactivation of this enzyme in the presence of 3-chlorocatechol was observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartels I., Knackmuss H. J., Reineke W. Suicide Inactivation of Catechol 2,3-Dioxygenase from Pseudomonas putida mt-2 by 3-Halocatechols. Appl Environ Microbiol. 1984 Mar;47(3):500–505. doi: 10.1128/aem.47.3.500-505.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayly R. C., Dagley S., Gibson D. T. The metabolism of cresols by species of Pseudomonas. Biochem J. 1966 Nov;101(2):293–301. doi: 10.1042/bj1010293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  4. Collins M. D., Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev. 1981 Jun;45(2):316–354. doi: 10.1128/mr.45.2.316-354.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crawford R. L. Novel pathway for degradation of protocatechuic acid in Bacillus species. J Bacteriol. 1975 Feb;121(2):531–536. doi: 10.1128/jb.121.2.531-536.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ewers J., Rubio M. A., Knackmuss H. J., Freier-Schröder D. Bacterial metabolism of 2,6-xylenol. Appl Environ Microbiol. 1989 Nov;55(11):2904–2908. doi: 10.1128/aem.55.11.2904-2908.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haigler B. E., Pettigrew C. A., Spain J. C. Biodegradation of mixtures of substituted benzenes by Pseudomonas sp. strain JS150. Appl Environ Microbiol. 1992 Jul;58(7):2237–2244. doi: 10.1128/aem.58.7.2237-2244.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hopper D. J., Taylor D. G. Pathways for the degradation of m-cresol and p-cresol by Pseudomonas putida. J Bacteriol. 1975 Apr;122(1):1–6. doi: 10.1128/jb.122.1.1-6.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kersten P. J., Chapman P. J., Dagley S. Enzymatic release of halogens or methanol from some substituted protocatechuic acids. J Bacteriol. 1985 May;162(2):693–697. doi: 10.1128/jb.162.2.693-697.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klecka G. M., Gibson D. T. Inhibition of catechol 2,3-dioxygenase from Pseudomonas putida by 3-chlorocatechol. Appl Environ Microbiol. 1981 May;41(5):1159–1165. doi: 10.1128/aem.41.5.1159-1165.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Knackmuss H. J., Hellwig M. Utilization and cooxidation of chlorinated phenols by Pseudomonas sp. B 13. Arch Microbiol. 1978 Apr 27;117(1):1–7. doi: 10.1007/BF00689343. [DOI] [PubMed] [Google Scholar]
  12. McIntire W., Hopper D. J., Singer T. P. p-Cresol methylhydroxylase. Assay and general properties. Biochem J. 1985 Jun 1;228(2):325–335. doi: 10.1042/bj2280325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ono K., Nozaki M., Hayaishi O. Purification and some properties of protocatechuate 4,5-dioxygenase. Biochim Biophys Acta. 1970 Nov 11;220(2):224–238. doi: 10.1016/0005-2744(70)90008-2. [DOI] [PubMed] [Google Scholar]
  14. Pettigrew C. A., Haigler B. E., Spain J. C. Simultaneous biodegradation of chlorobenzene and toluene by a Pseudomonas strain. Appl Environ Microbiol. 1991 Jan;57(1):157–162. doi: 10.1128/aem.57.1.157-162.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rojo F., Pieper D. H., Engesser K. H., Knackmuss H. J., Timmis K. N. Assemblage of ortho cleavage route for simultaneous degradation of chloro- and methylaromatics. Science. 1987 Dec 4;238(4832):1395–1398. doi: 10.1126/science.3479842. [DOI] [PubMed] [Google Scholar]
  16. Rudolphi A., Tschech A., Fuchs G. Anaerobic degradation of cresols by denitrifying bacteria. Arch Microbiol. 1991;155(3):238–248. doi: 10.1007/BF00252207. [DOI] [PubMed] [Google Scholar]
  17. STANIER R. Y., INGRAHAM J. L. Protocatechuic acid oxidase. J Biol Chem. 1954 Oct;210(2):799–808. [PubMed] [Google Scholar]
  18. Veys A., Callewaert W., Waelkens E., Van den Abbeele K. Application of gas-liquid chromatography to the routine identification of nonfermenting gram-negative bacteria in clinical specimens. J Clin Microbiol. 1989 Jul;27(7):1538–1542. doi: 10.1128/jcm.27.7.1538-1542.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wieser M., Eberspächer J., Vogler B., Lingens F. Metabolism of 4-chlorophenol by Azotobacter sp. GP1: structure of the meta cleavage product of 4-chlorocatechol. FEMS Microbiol Lett. 1994 Feb 1;116(1):73–78. doi: 10.1111/j.1574-6968.1994.tb06678.x. [DOI] [PubMed] [Google Scholar]

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