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. 1987 Apr;53(4):651–654. doi: 10.1128/aem.53.4.651-654.1987

Isolation and Some Properties of a β-d-Xylosidase from Clostridium acetobutylicum ATCC 824

Song F Lee 1, Cecil W Forsberg 1,*
PMCID: PMC203730  PMID: 16347313

Abstract

A β-d-xylosidase from C. acetobutylicum ATCC 824 was purified by column chromatography on CM-Sepharose, hydroxylapatite, Phenyl Sepharose, and Sephadex G-200. The enzyme had an apparent molecular weight of 224,000 as estimated by gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the enzyme consisted of two subunits of 85,000 and one subunit of 63,000 daltons. It exhibited optimal activity at pH 6.0 to 6.5 and 45°C. the enzyme had an isoelectric point of 5.85. It hydrolyzed p-nitrophenylxyloside readily with a Km of 3.7 mM. The enzyme hydrolyzed xylo-oligosaccharides with chain lengths of 2 to 6 units by cleaving a single xylose from the chain end. It showed little or no activity against xylan, carboxymethyl cellulose, and other p-nitrophenylglycosides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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