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. 1987 Jul;53(7):1632–1637. doi: 10.1128/aem.53.7.1632-1637.1987

Bacteriophages active against Bacteroides fragilis in sewage-polluted waters.

C Tartera 1, J Jofre 1
PMCID: PMC203922  PMID: 3662510

Abstract

Twelve strains of different Bacteroides species were tested for their efficiency of detection of bacteriophages from sewage. The host range of several isolated phages was investigated. The results indicated that there was a high degree of strain specificity. Then, by using Bacteroides fragilis HSP 40 as the host, which proved to be the most efficient for the detection of phages, feces from humans and several animal species and raw sewage, river water, water from lagoons, seawater, groundwater, and sediments were tested for the presence of bacteriophages that were active against B. fragilis HSP 40. Phages were detected in feces of 10% of the human fecal samples tested and was never detected in feces of the other animal species studied. Moreover, bacteriophages were always recovered from sewage and sewage-polluted samples of waters and sediments, but not from nonpolluted samples. The titers recovered were dependent on the degree of pollution in analyzed waters and sediments.

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Selected References

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  1. Agbalika F., Hartemann P., Foliguet J. M. Trypsin-treated Ma-104: a sensitive cell line for isolating enteric viruses from environmental samples. Appl Environ Microbiol. 1984 Feb;47(2):378–380. doi: 10.1128/aem.47.2.378-380.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg G., Dahling D. R., Brown G. A., Berman D. Validity of fecal coliforms, total coliforms, and fecal streptococci as indicators of viruses in chlorinated primary sewage effluents. Appl Environ Microbiol. 1978 Dec;36(6):880–884. doi: 10.1128/aem.36.6.880-884.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Booth S. J., Van Tassell R. L., Johnson J. L., Wilkins T. D. Bacteriophages of Bacteroides. Rev Infect Dis. 1979 Mar-Apr;1(2):325–336. doi: 10.1093/clinids/1.2.325. [DOI] [PubMed] [Google Scholar]
  4. Cabelli V. J., Dufour A. P., McCabe L. J., Levin M. A. Swimming-associated gastroenteritis and water quality. Am J Epidemiol. 1982 Apr;115(4):606–616. doi: 10.1093/oxfordjournals.aje.a113342. [DOI] [PubMed] [Google Scholar]
  5. Cliver D. O. Virus association with wastewater solids. Environ Lett. 1975;10(3):215–223. doi: 10.1080/00139307509435823. [DOI] [PubMed] [Google Scholar]
  6. Cooper S. W., Szymczak E. G., Jacobus N. V., Tally F. P. Differentiation of Bacteroides ovatus and Bacteroides thetaiotaomicron by means of bacteriophage. J Clin Microbiol. 1984 Dec;20(6):1122–1125. doi: 10.1128/jcm.20.6.1122-1125.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dhillon T. S., Chan Y. S., Sun S. M., Chau W. S. Distribution of coliphages in Hong Kong sewage. Appl Microbiol. 1970 Aug;20(2):187–191. doi: 10.1128/am.20.2.187-191.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dhillon T. S., Dhillon E. K., Chau H. C., Li W. K., Tsang A. H. Studies on bacteriophage distribution: virulent and temperate bacteriophage content of mammalian feces. Appl Environ Microbiol. 1976 Jul;32(1):68–74. doi: 10.1128/aem.32.1.68-74.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dhillon T. S., Dhillon E. K. Studies on bacteriophage distribution. II. Isolation and host rage based classification of phages active on three species of Enterobacteriaceae. Jpn J Microbiol. 1972 Jul;16(4):297–306. doi: 10.1111/j.1348-0421.1972.tb00662.x. [DOI] [PubMed] [Google Scholar]
  10. Gerba C. P., Goyal S. M., LaBelle R. L., Cech I., Bodgan G. F. Failure of indicator bacteria to reflect the occurrence of enteroviruses in marine waters. Am J Public Health. 1979 Nov;69(11):1116–1119. doi: 10.2105/ajph.69.11.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Goyal S. M., Adams W. N., O'Malley M. L., Lear D. W. Human pathogenic viruses at sewage sludge disposal sites in the Middle Atlantic region. Appl Environ Microbiol. 1984 Oct;48(4):758–763. doi: 10.1128/aem.48.4.758-763.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Havelaar A. H., Hogeboom W. M. Factors affecting the enumeration of coliphages in sewage and sewage-polluted waters. Antonie Van Leeuwenhoek. 1983 Nov;49(4-5):387–397. doi: 10.1007/BF00399318. [DOI] [PubMed] [Google Scholar]
  13. Hejkal T. W., Smith E. M., Gerba C. P. Seasonal occurrence of rotavirus in sewage. Appl Environ Microbiol. 1984 Mar;47(3):588–590. doi: 10.1128/aem.47.3.588-590.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Irving L. G., Smith F. A. One-year survey of enteroviruses, adenoviruses, and reoviruses isolated from effluent at an activated-sludge purification plant. Appl Environ Microbiol. 1981 Jan;41(1):51–59. doi: 10.1128/aem.41.1.51-59.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kai M., Watanabe S., Furuse K., Ozawa A. Bacteroides bacteriophages isolated from human feces. Microbiol Immunol. 1985;29(9):895–899. doi: 10.1111/j.1348-0421.1985.tb00891.x. [DOI] [PubMed] [Google Scholar]
  16. Keller R., Traub N. The characterization of Bacteroides fragilis bacteriophage recovered from animal sera: observations on the nature of bacteroides phage carrier cultures. J Gen Virol. 1974 Jul;24(1):179–189. doi: 10.1099/0022-1317-24-1-179. [DOI] [PubMed] [Google Scholar]
  17. Kott Y. Estimation of low numbers of Escherichia coli bacteriophage by use of the most probable number method. Appl Microbiol. 1966 Mar;14(2):141–144. doi: 10.1128/am.14.2.141-144.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nacescu N., Brandis H., Werner H. Isolierung von zwei Bacteroides fragilis-Phagen aus Abwasser und Nachweis lysogener B. fragilis-Stämme. Zentralbl Bakteriol Orig A. 1972 Apr;219(4):522–529. [PubMed] [Google Scholar]
  19. Payment P., Ayache R., Trudel M. A survey of enteric viruses in domestic sewage. Can J Microbiol. 1983 Jan;29(1):111–119. doi: 10.1139/m83-018. [DOI] [PubMed] [Google Scholar]
  20. Primrose S. B., Seeley N. D., Logan K. B., Nicolson J. W. Methods for studying aquatic bacteriophage ecology. Appl Environ Microbiol. 1982 Mar;43(3):694–701. doi: 10.1128/aem.43.3.694-701.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Salyers A. A. Bacteroides of the human lower intestinal tract. Annu Rev Microbiol. 1984;38:293–313. doi: 10.1146/annurev.mi.38.100184.001453. [DOI] [PubMed] [Google Scholar]
  22. Sattar S. A., Westwood J. C. Comparison of four eluents in the recovery of indigenous viruses from raw sludge. Can J Microbiol. 1976 Oct;22(10):1586–1589. doi: 10.1139/m76-233. [DOI] [PubMed] [Google Scholar]
  23. Simková A., Cervenka J. Coliphages as ecological indicators of enteroviruses in various water systems. Bull World Health Organ. 1981;59(4):611–618. [PMC free article] [PubMed] [Google Scholar]
  24. Stetler R. E., Ward R. L., Waltrip S. C. Enteric virus and indicator bacteria levels in a water treatment system modified to reduce trihalomethane production. Appl Environ Microbiol. 1984 Feb;47(2):319–324. doi: 10.1128/aem.47.2.319-324.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tomás J., Regué M., Parés R., Jofre J., Kay W. W. P1 bacteriophage and tellurite sensitivity in Klebsiella pneumoniae and Escherichia coli. Can J Microbiol. 1984 Jun;30(6):830–836. doi: 10.1139/m84-127. [DOI] [PubMed] [Google Scholar]
  26. Wellings F. M., Lewis A. L., Mountain C. W., Pierce L. V. Demonstration of virus in groundwater after effluent discharge onto soil. Appl Microbiol. 1975 Jun;29(6):751–757. doi: 10.1128/am.29.6.751-757.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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