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. 1987 Aug;53(8):1902–1906. doi: 10.1128/aem.53.8.1902-1906.1987

Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum.

M D Savage 1, Z G Wu 1, S L Daniel 1, L L Lundie Jr 1, H L Drake 1
PMCID: PMC204022  PMID: 3116936

Abstract

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO----CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; YH2 was considerably lower than YCO. Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

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Selected References

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