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. 1993 Apr;175(8):2458–2461. doi: 10.1128/jb.175.8.2458-2461.1993

Tyrosine phosphate in a- and b-type flagellins of Pseudomonas aeruginosa.

K Kelly-Wintenberg 1, S L South 1, T C Montie 1
PMCID: PMC204538  PMID: 7682214

Abstract

Both a- and b-type purified flagellins from a number of Pseudomonas aeruginosa strains grown in radiolabeled phosphate were shown to be phosphorylated. Analysis of partial acid-hydrolyzed flagellar filaments revealed that 32Pi was in phosphotyrosine. Three 32P-phosphopeptides apparently are common to a- and b-type flagellins, but a fourth peptide was found only in b-type hydrolysates. P. aeruginosa PAK flagellin, containing only two tyrosines, both in the variable region, was readily labeled and gave the same peptide pattern as flagellins containing additional tyrosines. Data showing that a- and b-type flagellins gave positive immunoblots with antiphosphotyrosine monoclonal antibody and that release of P(i) by alkaline phosphatase occurred indicated that unmodified tyrosine phosphate exists in flagellin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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