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. 1990 Mar;172(3):1271–1275. doi: 10.1128/jb.172.3.1271-1275.1990

Protein content and enzyme activities in methanol- and acetate-grown Methanosarcina thermophila.

P E Jablonski 1, A A DiMarco 1, T A Bobik 1, M C Cabell 1, J G Ferry 1
PMCID: PMC208594  PMID: 2307649

Abstract

The cell extract protein content of acetate- and methanol-grown Methanosarcina thermophila TM-1 was examined by two-dimensional polyacrylamide gel electrophoresis. More than 100 mutually exclusive spots were present in acetate- and methanol-grown cells. Spots corresponding to acetate kinase, phosphotransacetylase, and the five subunits of the carbon monoxide dehydrogenase complex were identified in acetate-grown cells. Activities of formylmethanofuran dehydrogenase, formylmethanofuran:tetrahydromethanopterin formyltransferase, 5,10-methenyltetrahydromethanopterin cyclohydrolase, methylene tetrahydromethanopterin:coenzyme F420 oxidoreductase, formate dehydrogenase, and carbonic anhydrase were examined in acetate- and methanol-grown Methanosarcina thermophila. Levels of formyltransferase in either acetate- or methanol-grown Methanosarcina thermophila were approximately half the levels detected in H2-CO2-grown Methanobacterium thermoautotrophicum. All other enzyme activities were significantly lower in acetate- and methanol-grown Methanosarcina thermophila.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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