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. 1989 Jan;171(1):577–580. doi: 10.1128/jb.171.1.577-580.1989

Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene.

A Matsuyama 1, H Yamamoto 1, E Nakano 1
PMCID: PMC209626  PMID: 2536666

Abstract

The Escherichia coli K-12 ackA gene, which encodes an acetate kinase, was cloned. The acetate kinase activities of ackA+ plasmid-containing strains were amplified 160- to 180-fold. The complete nucleotide sequence of the ackA gene was determined. It was deduced that the ackA gene coded for a protein of 400 amino acids with an Mr of 43,297. The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome.

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Selected References

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