Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Jun;170(6):2860–2865. doi: 10.1128/jb.170.6.2860-2865.1988

Evidence for endonucleolytic cleavages in decay of lacZ and lacI mRNAs.

M N Subbarao 1, D Kennell 1
PMCID: PMC211215  PMID: 2836375

Abstract

S1 nuclease mapping revealed lacZ mRNA molecules whose 5' and 3' ends were internal to the transcription start and consistent with cleavages at pyrimidine-adenosine bonds 20 to 50 nucleotides apart. With the net 5'-to-3' direction known, lacZ mRNA is probably degraded by sequential cleavages of naked mRNA at vulnerable sites exposed by transit of the last translating ribosome.

Full text

PDF
2864

Images in this article

2862Image
on p.2862
2863Image
on p.2863

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Büchel D. E., Gronenborn B., Müller-Hill B. Sequence of the lactose permease gene. Nature. 1980 Feb 7;283(5747):541–545. doi: 10.1038/283541a0. [DOI] [PubMed] [Google Scholar]
  2. Cannistraro V. J., Kennell D. Evidence that the 5' end of lac mRNA starts to decay as soon as it is synthesized. J Bacteriol. 1985 Feb;161(2):820–822. doi: 10.1128/jb.161.2.820-822.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cannistraro V. J., Kennell D. The 5' ends of Escherichia coli lac mRNA. J Mol Biol. 1985 Mar 20;182(2):241–248. doi: 10.1016/0022-2836(85)90342-0. [DOI] [PubMed] [Google Scholar]
  4. Cannistraro V. J., Subbarao M. N., Kennell D. Specific endonucleolytic cleavage sites for decay of Escherichia coli mRNA. J Mol Biol. 1986 Nov 20;192(2):257–274. doi: 10.1016/0022-2836(86)90363-3. [DOI] [PubMed] [Google Scholar]
  5. Cannistraro V. J., Wice B. M., Kennell D. E. Isolating and sequencing the predominant 5'-ends of a specific mRNA in cells. II. End-labeling and sequencing. J Biochem Biophys Methods. 1985 Aug;11(2-3):163–175. doi: 10.1016/0165-022x(85)90052-1. [DOI] [PubMed] [Google Scholar]
  6. Cone K. C., Sellitti M. A., Steege D. A. Lac repressor mRNA transcription terminates in vivo in the lac control region. J Biol Chem. 1983 Sep 25;258(18):11296–11304. [PubMed] [Google Scholar]
  7. Fowler A. V., Zabin I. Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence. J Biol Chem. 1978 Aug 10;253(15):5521–5525. [PubMed] [Google Scholar]
  8. Gottesman M., Oppenheim A., Court D. Retroregulation: control of gene expression from sites distal to the gene. Cell. 1982 Jul;29(3):727–728. doi: 10.1016/0092-8674(82)90434-2. [DOI] [PubMed] [Google Scholar]
  9. Hayashi M. N., Hayashi M., Müller U. R. Role for the J-F intercistronic region of bacteriophages phi X174 and G4 in stability of mRNA. J Virol. 1983 Oct;48(1):186–196. doi: 10.1128/jvi.48.1.186-196.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kalnins A., Otto K., Rüther U., Müller-Hill B. Sequence of the lacZ gene of Escherichia coli. EMBO J. 1983;2(4):593–597. doi: 10.1002/j.1460-2075.1983.tb01468.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kennell D., Riezman H. Transcription and translation initiation frequencies of the Escherichia coli lac operon. J Mol Biol. 1977 Jul;114(1):1–21. doi: 10.1016/0022-2836(77)90279-0. [DOI] [PubMed] [Google Scholar]
  12. Lim L. W., Kennell D. Models for decay of Escherichia coli lac messenger RNA and evidence for inactivating cleavages between its messages. J Mol Biol. 1979 Dec 5;135(2):369–390. doi: 10.1016/0022-2836(79)90442-x. [DOI] [PubMed] [Google Scholar]
  13. Maizels N. M. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585–3589. doi: 10.1073/pnas.70.12.3585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Richardson J. P., Ruteshouser E. C. rho factor-dependent transcription termination. Interference by a mutant rho. J Mol Biol. 1986 Jun 5;189(3):413–419. doi: 10.1016/0022-2836(86)90313-x. [DOI] [PubMed] [Google Scholar]
  15. Schindler D., Echols H. Retroregulation of the int gene of bacteriophage lambda: control of translation completion. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4475–4479. doi: 10.1073/pnas.78.7.4475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schneider E., Blundell M., Kennell D. Translation and mRNA decay. Mol Gen Genet. 1978 Apr 6;160(2):121–129. doi: 10.1007/BF00267473. [DOI] [PubMed] [Google Scholar]
  17. Sellitti M. A., Pavco P. A., Steege D. A. lac repressor blocks in vivo transcription of lac control region DNA. Proc Natl Acad Sci U S A. 1987 May;84(10):3199–3203. doi: 10.1073/pnas.84.10.3199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Steege D. A. 5'-Terminal nucleotide sequence of Escherichia coli lactose repressor mRNA: features of translational initiation and reinitiation sites. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4163–4167. doi: 10.1073/pnas.74.10.4163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zuker M., Stiegler P. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res. 1981 Jan 10;9(1):133–148. doi: 10.1093/nar/9.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES