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. 1988 Nov;170(11):5272–5278. doi: 10.1128/jb.170.11.5272-5278.1988

Chromosomal genes essential for stable maintenance of the mini-F plasmid in Escherichia coli.

H Niki 1, C Ichinose 1, T Ogura 1, H Mori 1, M Morita 1, M Hasegawa 1, N Kusukawa 1, S Hiraga 1
PMCID: PMC211601  PMID: 3053654

Abstract

We have isolated mutants of Escherichia coli which do not support stable maintenance of mini-F plasmids (delta ccd rep+ sop+). These host mutations, named hop, were classified into five linkage groups on the E. coli chromosome. Genetic analyses of these hop mutations by Hfr mating and P1 transduction showed their loci on the E. coli genetic map to be as follows: hopA in the gyrB-tnaA region, hopB in the bglB-oriC region, hopD between 8 and 15 min, and hopE in the argA-thyA region. Kinetics of stability of the sop+ and delta sop mini-F plasmids in these hop mutants suggest that the hopA mutants are defective in partitioning of mini-F rather than in plasmid replication. The hopB, hopC, and hopD mutants were partially defective in replication of mini-F. The physical structure of the plasmid DNA was normal in hopA, B, C, and D mutants. Large amounts of linear multimers of plasmid DNA accumulated in mutants of the fifth linkage group (hopE). None of the hop mutations in any linkage group affected the normal growth of cells.

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Selected References

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  1. Abeles A. L., Friedman S. A., Austin S. J. Partition of unit-copy miniplasmids to daughter cells. III. The DNA sequence and functional organization of the P1 partition region. J Mol Biol. 1985 Sep 20;185(2):261–272. doi: 10.1016/0022-2836(85)90402-4. [DOI] [PubMed] [Google Scholar]
  2. Austin S. J. Bacterial plasmids that carry two functional centromere analogs are stable and are partitioned faithfully. J Bacteriol. 1984 May;158(2):742–745. doi: 10.1128/jb.158.2.742-745.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Austin S., Abeles A. Partition of unit-copy miniplasmids to daughter cells. II. The partition region of miniplasmid P1 encodes an essential protein and a centromere-like site at which it acts. J Mol Biol. 1983 Sep 15;169(2):373–387. doi: 10.1016/s0022-2836(83)80056-4. [DOI] [PubMed] [Google Scholar]
  4. Austin S., Abeles A. The partition functions of P1, P7, and F miniplasmids. Basic Life Sci. 1985;30:215–226. doi: 10.1007/978-1-4613-2447-8_18. [DOI] [PubMed] [Google Scholar]
  5. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Biek D. P., Cohen S. N. Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli. J Bacteriol. 1986 Aug;167(2):594–603. doi: 10.1128/jb.167.2.594-603.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
  8. Cohen A., Clark A. J. Synthesis of linear plasmid multimers in Escherichia coli K-12. J Bacteriol. 1986 Jul;167(1):327–335. doi: 10.1128/jb.167.1.327-335.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cohen S. N., Chang A. C. Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc Natl Acad Sci U S A. 1973 May;70(5):1293–1297. doi: 10.1073/pnas.70.5.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cohen S. N., Chang A. C. Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol. 1977 Nov;132(2):734–737. doi: 10.1128/jb.132.2.734-737.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gerdes K., Larsen J. E., Molin S. Stable inheritance of plasmid R1 requires two different loci. J Bacteriol. 1985 Jan;161(1):292–298. doi: 10.1128/jb.161.1.292-298.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hayakawa Y., Murotsu T., Matsubara K. Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA. J Bacteriol. 1985 Jul;163(1):349–354. doi: 10.1128/jb.163.1.349-354.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Helsberg M., Eichenlaub R. Twelve 43-base-pair repeats map in a cis-acting region essential for partition of plasmid mini-F. J Bacteriol. 1986 Mar;165(3):1043–1045. doi: 10.1128/jb.165.3.1043-1045.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hiraga S., Jaffé A., Ogura T., Mori H., Takahashi H. F plasmid ccd mechanism in Escherichia coli. J Bacteriol. 1986 Apr;166(1):100–104. doi: 10.1128/jb.166.1.100-104.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hiraga S. Mechanisms of stable plasmid inheritance. Adv Biophys. 1986;21:91–103. doi: 10.1016/0065-227x(86)90016-x. [DOI] [PubMed] [Google Scholar]
  16. Hiraga S., Ogura T., Mori H., Tanaka M. Mechanisms essential for stable inheritance of mini-F plasmid. Basic Life Sci. 1985;30:469–487. doi: 10.1007/978-1-4613-2447-8_34. [DOI] [PubMed] [Google Scholar]
  17. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Jaffé A., Ogura T., Hiraga S. Effects of the ccd function of the F plasmid on bacterial growth. J Bacteriol. 1985 Sep;163(3):841–849. doi: 10.1128/jb.163.3.841-849.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Jamieson A. F., Bergquist P. L. Genetic mapping of chromosomal mutations affecting the replication of the F-factor of Escherichia coli. Mol Gen Genet. 1976 Oct 18;148(2):221–223. doi: 10.1007/BF00268388. [DOI] [PubMed] [Google Scholar]
  20. Kline B. C. A review of mini-F plasmid maintenance. Plasmid. 1985 Jul;14(1):1–16. doi: 10.1016/0147-619x(85)90027-7. [DOI] [PubMed] [Google Scholar]
  21. Kusukawa N., Mori H., Kondo A., Hiraga S. Partitioning of the F plasmid: overproduction of an essential protein for partition inhibits plasmid maintenance. Mol Gen Genet. 1987 Jul;208(3):365–372. doi: 10.1007/BF00328125. [DOI] [PubMed] [Google Scholar]
  22. Lane D., Rothenbuehler R., Merrillat A. M., Aiken C. Analysis of the F plasmid centromere. Mol Gen Genet. 1987 May;207(2-3):406–412. doi: 10.1007/BF00331608. [DOI] [PubMed] [Google Scholar]
  23. Meacock P. A., Cohen S. N. Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell. 1980 Jun;20(2):529–542. doi: 10.1016/0092-8674(80)90639-x. [DOI] [PubMed] [Google Scholar]
  24. Miki T., Chang Z. T., Horiuchi T. Control of cell division by sex factor F in Escherichia coli. II. Identification of genes for inhibitor protein and trigger protein on the 42.84-43.6 F segment. J Mol Biol. 1984 Apr 25;174(4):627–646. doi: 10.1016/0022-2836(84)90087-1. [DOI] [PubMed] [Google Scholar]
  25. Miller C. A., Tucker W. T., Meacock P. A., Gustafsson P., Cohen S. N. Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101. Gene. 1983 Oct;24(2-3):309–315. doi: 10.1016/0378-1119(83)90091-4. [DOI] [PubMed] [Google Scholar]
  26. Mori H., Kondo A., Ohshima A., Ogura T., Hiraga S. Structure and function of the F plasmid genes essential for partitioning. J Mol Biol. 1986 Nov 5;192(1):1–15. doi: 10.1016/0022-2836(86)90459-6. [DOI] [PubMed] [Google Scholar]
  27. Murotsu T., Matsubara K., Sugisaki H., Takanami M. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene. 1981 Nov;15(2-3):257–271. doi: 10.1016/0378-1119(81)90135-9. [DOI] [PubMed] [Google Scholar]
  28. Ogura T., Hiraga S. Mini-F plasmid genes that couple host cell division to plasmid proliferation. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4784–4788. doi: 10.1073/pnas.80.15.4784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ogura T., Hiraga S. Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition. Cell. 1983 Feb;32(2):351–360. doi: 10.1016/0092-8674(83)90454-3. [DOI] [PubMed] [Google Scholar]
  30. Phua S. H., Bergquist P. L., Lane H. E. Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis. Mol Gen Genet. 1982;188(2):353–355. doi: 10.1007/BF00332700. [DOI] [PubMed] [Google Scholar]
  31. Projan S. J., Carleton S., Novick R. P. Determination of plasmid copy number by fluorescence densitometry. Plasmid. 1983 Mar;9(2):182–190. doi: 10.1016/0147-619x(83)90019-7. [DOI] [PubMed] [Google Scholar]

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