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. 1971 Mar;69(1):73–82. doi: 10.1017/s0022172400021264

Investigation into rabbit infusion media for the growth of Mycoplasma gallisepticum antigens for inoculation into rabbits

Janet M Bradbury, F T W Jordan
PMCID: PMC2130842  PMID: 5291753

Abstract

Serum cholesterol in rabbits was elevated, by intravenous inoculation of 20% Tween 80, to amounts thought to have been sufficient to support growth of Mycoplasma gallisepticum when 20% of such serum was added to rabbit infusion broth.

However, better growth of the organism was obtained with a supplement of 5% rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARGAMAN M., RAZIN S. CHOLESTEROL AND CHOLESTEROL ESTERS IN MYCOPLASMA. J Gen Microbiol. 1965 Jan;38:153–160. doi: 10.1099/00221287-38-1-153. [DOI] [PubMed] [Google Scholar]
  2. BUTLER M., KNIGHT B. C. The survival of washed suspensions of Mycoplasma. J Gen Microbiol. 1960 Apr;22:470–477. doi: 10.1099/00221287-22-2-470. [DOI] [PubMed] [Google Scholar]
  3. EDWARD D. G., FITZGERALD W. A. Cholesterol in the growth of organisms of the pleuropneumonia group. J Gen Microbiol. 1951 Aug;5(3):576–586. doi: 10.1099/00221287-5-3-576. [DOI] [PubMed] [Google Scholar]
  4. EDWARD D. G., FITZGERALD W. A. The isolation of organisms of the pleuropneumonia group from dogs. J Gen Microbiol. 1951 Aug;5(3):566–575. doi: 10.1099/00221287-5-3-566. [DOI] [PubMed] [Google Scholar]
  5. EDWARD D. G., FREUNDT E. A. The classification and nomenclature of organisms of the pleuropneumonia group. J Gen Microbiol. 1956 Feb;14(1):197–207. doi: 10.1099/00221287-14-1-197. [DOI] [PubMed] [Google Scholar]
  6. ENNY G. E., GRAYSTON J. T. EATON PLEUROPNEUMONIA-LIKE ORGANISM (MYCOPLASMA PNEUMONIAE) COMPLEMENT-FIXING ANTIGEN: EXTRACTION WITH ORGANIC SOLVENTS. J Immunol. 1965 Jul;95:19–25. [PubMed] [Google Scholar]
  7. Fernald G. W., Clyde W. A., Jr, Denny F. W. Factors influencing growth inhibition of Mycoplasma pneumoniae by immune sera. Proc Soc Exp Biol Med. 1967 Oct;126(1):161–166. doi: 10.3181/00379727-126-32391. [DOI] [PubMed] [Google Scholar]
  8. Friedrich H. Zur Anwendung des Stoffwechselhemmtests (MI-test) bei Mycoplasma gallisepticum. Dtsch Tierarztl Wochenschr. 1970 Jan 1;77(1):12–14. [PubMed] [Google Scholar]
  9. Jordan F. T., Kulasegaram P. Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum. J Comp Pathol. 1968 Oct;78(4):407–414. doi: 10.1016/0021-9975(68)90038-8. [DOI] [PubMed] [Google Scholar]
  10. KELLNER A., CORRELL J. W., LADD A. T. Sustained hyperlipemia induced in rabbits by means of intravenously injected surface-active agents. J Exp Med. 1951 Apr 1;93(4):373–384. doi: 10.1084/jem.93.4.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MORAN P. A. Another test for heterogeneity of host resistance in dilution assays. J Hyg (Lond) 1958 Sep;56(3):319–322. doi: 10.1017/s0022172400037815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MORAN P. A. The dilution assay of viruses. J Hyg (Lond) 1954 Jun;52(2):189–193. doi: 10.1017/s002217240002739x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RODWELL A. W., ABBOT A. The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of Mycoplasma mycoides. J Gen Microbiol. 1961 Jun;25:201–214. doi: 10.1099/00221287-25-2-201. [DOI] [PubMed] [Google Scholar]
  14. RODWELL A. W. THE STEROID GROWTH-REQUIREMENT OF MYCOPLASMA MYCOIDES. J Gen Microbiol. 1963 Jul;32:91–101. doi: 10.1099/00221287-32-1-91. [DOI] [PubMed] [Google Scholar]
  15. SEARCY R. L., BERGQUIST L. M. A new color reaction for the quantitation of serum cholesterol. Clin Chim Acta. 1960 Mar;5:192–199. doi: 10.1016/0009-8981(60)90035-8. [DOI] [PubMed] [Google Scholar]
  16. SMITH P. F., LECCE J. G., LYNN R. J. A lipoprotein as a growth factor for cortain pleuropneumonialike organisms. J Bacteriol. 1954 Nov;68(5):627–633. doi: 10.1128/jb.68.5.627-633.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SMITH P. F. Nutritional requirements of PPLO and their relation to metabolic function. Ann N Y Acad Sci. 1960 Jan 15;79:508–520. doi: 10.1111/j.1749-6632.1960.tb42717.x. [DOI] [PubMed] [Google Scholar]
  18. Smith S. C., Dunlop W. R., Strout R. G. Effect of culture medium on antigenic structure of mycoplasma. Avian Dis. 1966 May;10(2):173–176. [PubMed] [Google Scholar]
  19. TAYLOR-ROBINSON D., SOMERSON N. L., TURNER H. C., CHANOCK R. M. SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION. J Bacteriol. 1963 Jun;85:1261–1273. doi: 10.1128/jb.85.6.1261-1273.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]

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