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. 1986 Nov;168(2):1010–1013. doi: 10.1128/jb.168.2.1010-1013.1986

Partition functions of unit-copy plasmids can stabilize the maintenance of plasmid pBR322 at low copy number.

S Austin, S Friedman, D Ludtke
PMCID: PMC213585  PMID: 3536844

Abstract

The maintenance of plasmid pBR322 is highly unstable in a polA12 strain of Escherichia coli at 29 degrees C due to severely reduced copy number. Under these conditions, introduction of the par (partition) locus of plasmid P1 or the par (sop) region of F into pBR322 stabilizes it. A region with similar activity was detected in the P7 plasmid. The activity of the P1 par locus was dependent on the P1 parA gene product and was sensitive to par-specified incompatibility.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abeles A. L., Friedman S. A., Austin S. J. Partition of unit-copy miniplasmids to daughter cells. III. The DNA sequence and functional organization of the P1 partition region. J Mol Biol. 1985 Sep 20;185(2):261–272. doi: 10.1016/0022-2836(85)90402-4. [DOI] [PubMed] [Google Scholar]
  2. Austin S., Abeles A. Partition of unit-copy miniplasmids to daughter cells. I. P1 and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equipartition. J Mol Biol. 1983 Sep 15;169(2):353–372. doi: 10.1016/s0022-2836(83)80055-2. [DOI] [PubMed] [Google Scholar]
  3. Austin S., Abeles A. Partition of unit-copy miniplasmids to daughter cells. II. The partition region of miniplasmid P1 encodes an essential protein and a centromere-like site at which it acts. J Mol Biol. 1983 Sep 15;169(2):373–387. doi: 10.1016/s0022-2836(83)80056-4. [DOI] [PubMed] [Google Scholar]
  4. Austin S., Wierzbicki A. Two mini-F-encoded proteins are essential for equipartition. Plasmid. 1983 Jul;10(1):73–81. doi: 10.1016/0147-619x(83)90059-8. [DOI] [PubMed] [Google Scholar]
  5. Chattoraj D. K., Snyder K. M., Abeles A. L. P1 plasmid replication: multiple functions of RepA protein at the origin. Proc Natl Acad Sci U S A. 1985 May;82(9):2588–2592. doi: 10.1073/pnas.82.9.2588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frame R., Bishop J. O. The number of sex-factors per chromosome in Escherichia coli. Biochem J. 1971 Jan;121(1):93–103. doi: 10.1042/bj1210093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hayakawa Y., Murotsu T., Matsubara K. Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA. J Bacteriol. 1985 Jul;163(1):349–354. doi: 10.1128/jb.163.1.349-354.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hedges R. W., Jacob A. E., Barth P. T., Grinter N. J. Compatibility properties of P1 and PhiAMP prophages. Mol Gen Genet. 1975 Dec 1;141(3):263–267. doi: 10.1007/BF00341804. [DOI] [PubMed] [Google Scholar]
  9. Iida S., Arber W. Multiple physical differences in the genome structure of functionally related bacteriophages P1 and P7. Mol Gen Genet. 1979 Jun 20;173(3):249–261. doi: 10.1007/BF00268635. [DOI] [PubMed] [Google Scholar]
  10. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  11. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  12. Monk M., Kinross J. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J Bacteriol. 1972 Mar;109(3):971–978. doi: 10.1128/jb.109.3.971-978.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ogura T., Hiraga S. Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition. Cell. 1983 Feb;32(2):351–360. doi: 10.1016/0092-8674(83)90454-3. [DOI] [PubMed] [Google Scholar]
  14. Prentki P., Chandler M., Caro L. Replication of prophage P1 during the cell cycle of Escherichia coli. Mol Gen Genet. 1977 Mar 28;152(1):71–76. doi: 10.1007/BF00264942. [DOI] [PubMed] [Google Scholar]
  15. Rokeach L. A., Søgaard-Andersen L., Molin S. Two functions of the E protein are key elements in the plasmid F replication control system. J Bacteriol. 1985 Dec;164(3):1262–1270. doi: 10.1128/jb.164.3.1262-1270.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stoker N. G., Fairweather N. F., Spratt B. G. Versatile low-copy-number plasmid vectors for cloning in Escherichia coli. Gene. 1982 Jun;18(3):335–341. doi: 10.1016/0378-1119(82)90172-x. [DOI] [PubMed] [Google Scholar]
  17. Trawick J. D., Kline B. C. A two-stage molecular model for control of mini-F replication. Plasmid. 1985 Jan;13(1):59–69. doi: 10.1016/0147-619x(85)90056-3. [DOI] [PubMed] [Google Scholar]

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