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. 1984 Nov;160(2):662–667. doi: 10.1128/jb.160.2.662-667.1984

Mechanism of acquisition of chromosomal markers by plasmids in Haemophilus influenzae.

J K Setlow, E Cabrera-Juárez, K Griffin
PMCID: PMC214786  PMID: 6094481

Abstract

The hybrid plasmid pNov1 readily acquired genetic information from the chromosome of wild-type, but not rec-2, cells. Most of the recombination had taken place 1 h after entrance of the plasmid into the cell, as judged by transformation of rec-2 by lysates made from wild-type cells exposed to pNov1. Measurement of physical transfer from radioactively labeled cellular DNA to plasmids recombining in wild-type cells failed, since there was little more radioactivity in plasmids from such cells than from labeled rec-2 recipients, in which no recombination took place. EcoRI digestion of pNov1 divided the DNA into a 1.7-kilobase-pair fragment containing the novobiocin resistance marker and a 13-kilobase-pair fragment containing all of the original vector and considerable portions homologous to the chromosome. Transformation by the large fragment alone resulted in a plasmid the size of the original pNov1. Our hypothesis to explain the data is that genetic transfer from chromosome to plasmid took place by a copy choice mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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