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. 1984 Nov;160(2):808–811. doi: 10.1128/jb.160.2.808-811.1984

Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus.

A R Datta, J B Kaper, A M MacQuillan
PMCID: PMC214813  PMID: 6094492

Abstract

Two cosmid cloning vectors containing lambda cos sequences and a 42-base-pair multipurpose cloning sequence were constructed. pAD22 also contains a 1.4-kilobase TRP-ARS fragment from Saccharomyces cerevisiae. These cosmids transformed Escherichia coli and S. cerevisiae cells and could be mobilized into Vibrio parahaemolyticus strains with a conjugative plasmid, pRK2013. The cosmid pAD22 was genetically and structurally stable during passage through V. parahaemolyticus and E. coli strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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