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. 1984 Jan;157(1):283–290. doi: 10.1128/jb.157.1.283-290.1984

Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae.

A B Futcher, B S Cox
PMCID: PMC215164  PMID: 6361000

Abstract

The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, high copy number is associated with low instability; (iii) it is difficult to reconcile this variability with a strict and direct system of copy number control; (iv) instabilities are much higher than expected from random partition and the observed copy numbers: this may imply partition which is less efficient than random. Even so, (v) the partitioning of 2-micron circle-like plasmids is more efficient than that of ARS-based plasmids, which hints at the existence of a system for the (inefficient) distribution of 2-micron circles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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