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. 1981 Nov;148(2):724–727. doi: 10.1128/jb.148.2.724-727.1981

Membrane and cytoplasmic nitrate reductase of Staphylococcus aureus and application of crossed immunoelectrophoresis.

K A Burke, A E Brown, J Lascelles
PMCID: PMC216261  PMID: 7197672

Abstract

Specific antiserum to the membrane nitrate reductase of Staphylococcus aureus was derived from immunoprecipitates on crossed immunoelectrophoresis plates. Analysis of the cytoplasmic and membrane forms of the enzyme in cells grown with nitrate and azide indicated their identity, and in each case, the major subunit, Mr 140,000, was converted by trypsin to a polypeptide, Mr 112,000, without loss of enzyme activity or immunological reactivity.

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Selected References

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