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. 1981 Nov;148(2):724–727. doi: 10.1128/jb.148.2.724-727.1981

Membrane and cytoplasmic nitrate reductase of Staphylococcus aureus and application of crossed immunoelectrophoresis.

K A Burke, A E Brown, J Lascelles
PMCID: PMC216261  PMID: 7197672

Abstract

Specific antiserum to the membrane nitrate reductase of Staphylococcus aureus was derived from immunoprecipitates on crossed immunoelectrophoresis plates. Analysis of the cytoplasmic and membrane forms of the enzyme in cells grown with nitrate and azide indicated their identity, and in each case, the major subunit, Mr 140,000, was converted by trypsin to a polypeptide, Mr 112,000, without loss of enzyme activity or immunological reactivity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burke K. A., Lascelles J. Nitrate reductase activity in heme-deficient mutants of Staphylococcus aureus. J Bacteriol. 1976 Apr;126(1):225–231. doi: 10.1128/jb.126.1.225-231.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burke K. A., Lascelles J. Nitrate reductase system in Staphylococcus aureus wild type and mutants. J Bacteriol. 1975 Jul;123(1):308–316. doi: 10.1128/jb.123.1.308-316.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burke K. A., Lascelles J. Partial purification and some properties of the Staphylococcus aureus cytoplasmic nitrate reductase. J Bacteriol. 1979 Jul;139(1):120–125. doi: 10.1128/jb.139.1.120-125.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hackett C. S., MacGregor C. H. Synthesis and degradation of nitrate reductase in Escherichia coli. J Bacteriol. 1981 Apr;146(1):352–359. doi: 10.1128/jb.146.1.352-359.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hederstedt L., Rutberg L. Biosynthesis and membrane binding of succinate dehydrogenase in Bacillus subtilis. J Bacteriol. 1980 Dec;144(3):941–951. doi: 10.1128/jb.144.3.941-951.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lascelles J. Heme-deficient mutants of Staphylococcus aureus. Methods Enzymol. 1979;56:172–178. doi: 10.1016/0076-6879(79)56019-4. [DOI] [PubMed] [Google Scholar]
  7. Lascelles J. sn-Glycerol-3-phosphate dehydrogenase and its interaction with nitrate reductase in wild-type and hem mutant strains of Staphylococcus aureus. J Bacteriol. 1978 Feb;133(2):621–625. doi: 10.1128/jb.133.2.621-625.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacGregor C. H. Biosynthesis of membrane-bound nitrate reductase in Escherichia coli: evidence for a soluble precursor. J Bacteriol. 1976 Apr;126(1):122–131. doi: 10.1128/jb.126.1.122-131.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Owen P., Salton M. R. Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3711–3715. doi: 10.1073/pnas.72.9.3711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rutberg B., Hederstedt L., Holmgren E., Rutberg L. Characterization of succinic dehydrogenase mutants of Bacillus subtilis by crossed immunoelectrophoresis. J Bacteriol. 1978 Oct;136(1):304–311. doi: 10.1128/jb.136.1.304-311.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith L. Bacterial cytochromes and their spectral characterization. Methods Enzymol. 1978;53:202–212. doi: 10.1016/s0076-6879(78)53025-5. [DOI] [PubMed] [Google Scholar]

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