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. 1982 Feb;149(2):499–507. doi: 10.1128/jb.149.2.499-507.1982

Kinetics of minichromosome replication in Escherichia coli B/r.

A C Leonard, J A Hucul, C E Helmstetter
PMCID: PMC216534  PMID: 7035432

Abstract

Replication control of the minichromosome pAL2 was found to differ from that of the chromosome in synchronously dividing populations of Escherichia coli B/r. Initiation of minichromosome replication took place at an increasing rate throughout synchronous growth. No coupling to initiation of chromosome replication was detected. Minichromosome replication was further examined in a dnaA5(Ts) temperature-sensitive initiation mutant. When cultures held at nonpermissive temperature (41 degrees C) for 60 min were shifted to permissive temperature (25 degrees C), initiation of both pAL2 and chromosome replication ensued in two waves spaced 25 to 35 min apart. Evidence is presented that minichromosomes terminate replication by passing slowly through a series of dimeric intermediate forms before reaching the closed circular monomeric form. The consequence of this slow passage as a rate-limiting step in the initiation reaction is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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