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. 1982 Feb;149(2):694–699. doi: 10.1128/jb.149.2.694-699.1982

Physical mapping and cloning of bacteriophage T4 anti-restriction endonuclease gene.

K Dharmalingam, H R Revel, E B Goldberg
PMCID: PMC216561  PMID: 6276366

Abstract

We have proposed that the ability of T4 to produce non-glucosylated progeny after a single cycle of growth on a galU rglA rglB+ mutant of Escherichia coli is due to the initiation of the rglB+ function by a phage-coded, anti-restriction endonuclease protein. Based on this hypothesis, we screened T4 deletion mutants for failure to give a burst in this host. The absence of an arn gene in phage mutants lacking the 55.5- to 58.4-kilobase region is verified by their inability to protect secondary infecting non-glucosylated phage from rglB-controlled cleavage. A functional arn gene was cloned on plasmid pBR325, and the 0.8-kilobase insert DNA was shown to be homologous to the DNA missing in the arn deletion phage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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