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. 1983 May;154(2):663–668. doi: 10.1128/jb.154.2.663-668.1983

Aldohexuronate transport system in Erwinia carotovora.

N Hugouvieux-Cotte-Pattat, Y Quesneau, J Robert-Baudouy
PMCID: PMC217514  PMID: 6841313

Abstract

The biochemical and physiological aspects of hexuronate transport in Erwinia carotovora were studied to approach the genetic regulation of the hexuronate degradative pathway in this bacterial species. An active transport system for glucuronate and galacturonate uptake exists in E. carotovora. The glucuronate entry reaction displayed saturation kinetics with an apparent Km of 0.05 mM (at 25 degrees C; pH 7). Galacturonate appeared to be a competitive inhibitor of glucuronate uptake with a Ki of 0.1 mM. Glucuronate permeation was not induced by glucuronate itself in wild-type strains. Galacturonate induced the uptake of glucuronate (about fivefold). The induced synthesis of the transport system was sensitive to catabolite repression by glucose. Mutants able to grow on glucuronate as the sole carbon source showed constitutive synthesis of the hexuronate transport system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collmer A., Bateman D. F. Impaired induction and self-catabolite repression of extracellular pectate lyase in Erwinia chrysanthemi mutants deficient in oligogalacturonide lyase. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3920–3924. doi: 10.1073/pnas.78.6.3920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Isolation of fusions between the lac genes and several genes of the exu regulon: analysis of their regulation, determination of the transcription direction of the uxaC-uxaA operon, in Escherichia coli K-12. Mol Gen Genet. 1981;182(2):279–287. doi: 10.1007/BF00269671. [DOI] [PubMed] [Google Scholar]
  3. Hugovieux-Cotte-Pattat N., Robert-Baudouy J. Regulation and transcription direction of exuR, a self-regulated repressor in escherichia coli K-12. J Mol Biol. 1982 Mar 25;156(1):221–228. doi: 10.1016/0022-2836(82)90468-5. [DOI] [PubMed] [Google Scholar]
  4. KILGORE W. W., STARR M. P. Catabolism of galacturonic and glucuronic acids by Erwinia carotovora. J Biol Chem. 1959 Sep;234:2227–2235. [PubMed] [Google Scholar]
  5. Kotoujansky A., Lemattre M., Boistard P. Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi. J Bacteriol. 1982 Apr;150(1):122–131. doi: 10.1128/jb.150.1.122-131.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lagarde A. E., Pouysségur J. M., Stoeber F. R. A transport system for 2-keto-3-deoxy-D-gluconate uptake in Escherichia coli K12. Biochemical and physiological studies in whole cells. Eur J Biochem. 1973 Jul 16;36(2):328–341. doi: 10.1111/j.1432-1033.1973.tb02917.x. [DOI] [PubMed] [Google Scholar]
  7. Nemoz G., Robert-Baudouy J., Stoeber F. Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli. J Bacteriol. 1976 Aug;127(2):706–718. doi: 10.1128/jb.127.2.706-718.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Portalier R., Robert-Baudouy J., Stoeber F. Regulation of Escherichia coli K-12 hexuronate system genes: exu regulon. J Bacteriol. 1980 Sep;143(3):1095–1107. doi: 10.1128/jb.143.3.1095-1107.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pouyssegur J., Stoeber F. Synthèse enzymatique du 2-céto-3-désoxy-D-gluconate. Bull Soc Chim Biol (Paris) 1970;52(12):1419–1428. [PubMed] [Google Scholar]
  10. Pouysségur J., Lagarde A. Système de transport du 2-céto-3-désoxy-gluconate chez E. coli K 12: localisation d'un gène de structure et de son opérateur. Mol Gen Genet. 1973 Mar 1;121(2):163–180. doi: 10.1007/BF00277530. [DOI] [PubMed] [Google Scholar]
  11. Robert-Baudouy J. M., Portalier R. C., Stoeber F. R. Régulation due métabolisme des hexuronates chez Escherichia coli K12. Modalités de l'induction des enzymes du système hexuronate. Eur J Biochem. 1974 Mar 15;43(1):1–15. doi: 10.1111/j.1432-1033.1974.tb03378.x. [DOI] [PubMed] [Google Scholar]
  12. Robert-Baudouy J. M., Stoeber F. R. Purification et propriétés de la D-mannonate hydrolyase d'Escherichia coli. Biochim Biophys Acta. 1973 Jun 6;309(2):473–485. doi: 10.1016/0005-2744(73)90045-4. [DOI] [PubMed] [Google Scholar]
  13. Robert-Baudouy J., Portalier R., Stoeber F. Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products. J Bacteriol. 1981 Jan;145(1):211–220. doi: 10.1128/jb.145.1.211-220.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Starr M. P., Chatterjee A. K. The genus Erwinia: enterobacteria pathogenic to plants and animals. Annu Rev Microbiol. 1972;26:389–426. doi: 10.1146/annurev.mi.26.100172.002133. [DOI] [PubMed] [Google Scholar]
  15. Stoeber F., Lagarde A., Nemoz G., Novel G., Novel M., Portalier R., Pouyssegur J., Robert-Baudouy J. Le métabolisme des hexuronides et des hexuronates chez Escherichia coli K 12: aspects physiologiques et et génétiques de sa régulation. Biochimie. 1974;56(2):199–213. doi: 10.1016/s0300-9084(74)80379-2. [DOI] [PubMed] [Google Scholar]
  16. Tsuyumu S. "Self-catabolite repression" of pectate lyase in Erwinia carotovora. J Bacteriol. 1979 Feb;137(2):1035–1036. doi: 10.1128/jb.137.2.1035-1036.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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