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. 1983 May;154(2):663–668. doi: 10.1128/jb.154.2.663-668.1983

Aldohexuronate transport system in Erwinia carotovora.

N Hugouvieux-Cotte-Pattat, Y Quesneau, J Robert-Baudouy
PMCID: PMC217514  PMID: 6841313

Abstract

The biochemical and physiological aspects of hexuronate transport in Erwinia carotovora were studied to approach the genetic regulation of the hexuronate degradative pathway in this bacterial species. An active transport system for glucuronate and galacturonate uptake exists in E. carotovora. The glucuronate entry reaction displayed saturation kinetics with an apparent Km of 0.05 mM (at 25 degrees C; pH 7). Galacturonate appeared to be a competitive inhibitor of glucuronate uptake with a Ki of 0.1 mM. Glucuronate permeation was not induced by glucuronate itself in wild-type strains. Galacturonate induced the uptake of glucuronate (about fivefold). The induced synthesis of the transport system was sensitive to catabolite repression by glucose. Mutants able to grow on glucuronate as the sole carbon source showed constitutive synthesis of the hexuronate transport system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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