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. 1983 Jul;155(1):196–202. doi: 10.1128/jb.155.1.196-202.1983

Ti plasmid and chromosomal ornithine catabolism genes of Agrobacterium tumefaciens C58.

C L Schardl, C I Kado
PMCID: PMC217669  PMID: 6305908

Abstract

The pTiC58 plasmid noc genes of Agrobacterium tumefaciens C58 code for nopaline oxidase (nocC), nopaline permease (nocP), the inducible periplasmic protein n1 (nocB), and a function(s) required for ornithine catabolism (nocA). In addition, strains C58 and Ach-5 of A. tumefaciens have chromosomal ornithine catabolism genes. The chromosomal orc gene codes for ornithine dehydrogenase. Strain C58 is normally orc, but orc+ mutants can be selected. We have characterized both chromosomal orc and pTiC58 nocA plasmid genes. Complementation of most chromosomal orc mutants by pTiC58 restored growth on both nopaline and L-ornithine but did not restore ornithine dehydrogenase activity. We conclude that ornithine is an intermediate of nopaline degradation and that the Ti plasmid and chromosome both code for ornithine-degradative enzymes. A model for nopaline catabolism is presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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