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. 1979 May;138(2):559–566. doi: 10.1128/jb.138.2.559-566.1979

Mutants of the mini-F plasmid pML31 thermosensitive in replication.

R Eichenlaub
PMCID: PMC218213  PMID: 374396

Abstract

Hydroxylamine mutagenesis was used for the induction of thermosensitive replication mutants of the mini-F plasmid pML31. Replication mutants were characterized by studying the segregation kinetics and the incorporation of [3H]-thymidine into plasmid deoxyribonucleic acid at the nonpermissive temperature. Based on these experiments two types of mutants could be distinguished. Mutants of type I are fast segregating with the kinetics expected if plasmid replication was blocked immediately. Double-label experiments showed a rapid shut-off of replication in these mutants at 42 degrees C. Mutants of type II segregate slower, showing only a partial inhibition of plasmid deoxyribonucleic acid synthesis at the nonpermissive temperature. The label incorporated at 42 degrees C was predominantly found in open circular plasmid molecules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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