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. 1979 Mar;137(3):1243–1252. doi: 10.1128/jb.137.3.1243-1252.1979

Enzymatic degradation of uracil-containing deoxyribonucleic acid. V. Survival of Escherichia coli and coliphages treated with sodium bisulfite.

R R Simmons, E C Friedberg
PMCID: PMC218307  PMID: 374345

Abstract

A number of mutants of Escherichia coli defective in the ung gene (structural gene for uracil-deoxyribonucleic acid [ura-DNA] glycosylase) are shown to be abnormally sensitive to treatment with sodium bisulfite when compared with congenic ung+ strains. These results provide further evidence that sodium bisulfite causes the deamination of cytosine to uracil in DNA and that ura-DNA glycosylase is required for the repair of U-G mispairs. The effect of the chemical is apparently selective with respect to base damage; coliphages containing cytosine in their DNA are inactivated by treatment with sodium bisulfite, whereas those containing hydroxymethylcytosine are not. ura-DNA glycosylase and the major apurinic-apyrimidinic endonuclease of E. coli may function in the same repair pathway, since the extent of inactivation of a congenic set of strains which are ung xth (structural gene for the major apurinic-apyrimidinic endonuclease of E. coli) or ung xth+ is the same.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brynolf K., Eliasson R., Reichard P. Formation of Okazaki fragments in polyoma DNA synthesis caused by misincorporation of uracil. Cell. 1978 Mar;13(3):573–580. doi: 10.1016/0092-8674(78)90330-6. [DOI] [PubMed] [Google Scholar]
  2. Cone R., Duncan J., Hamilton L., Friedberg E. C. Partial purification and characterization of a uracil DNA N-glycosidase from Bacillus subtilis. Biochemistry. 1977 Jul 12;16(14):3194–3201. doi: 10.1021/bi00633a024. [DOI] [PubMed] [Google Scholar]
  3. Da Roza R., Friedberg E. C., Duncan B. K., Warner H. R. Repair of nitrous acid damage to DNA in Escherichia coli. Biochemistry. 1977 Nov 1;16(22):4934–4939. doi: 10.1021/bi00641a030. [DOI] [PubMed] [Google Scholar]
  4. Duncan B. K., Rockstroh P. A., Warner H. R. Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase. J Bacteriol. 1978 Jun;134(3):1039–1045. doi: 10.1128/jb.134.3.1039-1045.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duncan J., Hamilton L., Friedberg E. C. Enzymatic degradation of uracil-containing DNA. II. Evidence for N-glycosidase and nuclease activities in unfractionated extracts of Bacillus subtilis. J Virol. 1976 Aug;19(2):338–345. doi: 10.1128/jvi.19.2.338-345.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedberg E. C., Ganesan A. K., Minton K. N-Glycosidase activity in extracts of Bacillus subtilis and its inhibition after infection with bacteriophage PBS2. J Virol. 1975 Aug;16(2):315–321. doi: 10.1128/jvi.16.2.315-321.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedberg E. C., King J. J. Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease. J Bacteriol. 1971 May;106(2):500–507. doi: 10.1128/jb.106.2.500-507.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gates F. T., 3rd, Linn S. Endonuclease V of Escherichia coli. J Biol Chem. 1977 Mar 10;252(5):1647–1653. [PubMed] [Google Scholar]
  9. Gates F. T., Linn S. Endonuclease from Escherichia coli that acts specifically upon duplex DNA damaged by ultraviolet light, osmium tetroxide, acid, or x-rays. J Biol Chem. 1977 May 10;252(9):2802–2807. [PubMed] [Google Scholar]
  10. Hayatsu H. Bisulfite modification of nucleic acids and their constituents. Prog Nucleic Acid Res Mol Biol. 1976;16:75–124. doi: 10.1016/s0079-6603(08)60756-4. [DOI] [PubMed] [Google Scholar]
  11. Hayatsu H., Wataya Y., Kai K., Iida S. Reaction of sodium bisulfite with uracil, cytosine, and their derivatives. Biochemistry. 1970 Jul 7;9(14):2858–2865. doi: 10.1021/bi00816a016. [DOI] [PubMed] [Google Scholar]
  12. Hochhauser S. J., Weiss B. Escherichia coli mutants deficient in deoxyuridine triphosphatase. J Bacteriol. 1978 Apr;134(1):157–166. doi: 10.1128/jb.134.1.157-166.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Isolation and characterization of a Bacillus subtilis mutant with a defective N-glycosidase activity for uracil-containing deoxyribonucleic acid. J Bacteriol. 1977 Aug;131(2):438–445. doi: 10.1128/jb.131.2.438-445.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuhnlein U., Lee B., Linn S. Human uracil DNA N-glycosidase: studies in normal and repair defective cultured fibroblasts. Nucleic Acids Res. 1978 Jan;5(1):117–125. doi: 10.1093/nar/5.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laval J. Two enzymes are required from strand incision in repair of alkylated DNA. Nature. 1977 Oct 27;269(5631):829–832. doi: 10.1038/269829a0. [DOI] [PubMed] [Google Scholar]
  16. Lindahl T. An N-glycosidase from Escherichia coli that releases free uracil from DNA containing deaminated cytosine residues. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3649–3653. doi: 10.1073/pnas.71.9.3649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lindahl T. Irreversible heat inactivation of transfer ribonucleic acids. J Biol Chem. 1967 Apr 25;242(8):1970–1973. [PubMed] [Google Scholar]
  18. Lindahl T., Ljungquist S., Siegert W., Nyberg B., Sperens B. DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli. J Biol Chem. 1977 May 25;252(10):3286–3294. [PubMed] [Google Scholar]
  19. Lindahl T. New class of enzymes acting on damaged DNA. Nature. 1976 Jan 1;259(5538):64–66. doi: 10.1038/259064a0. [DOI] [PubMed] [Google Scholar]
  20. Lindahl T., Nyberg B. Heat-induced deamination of cytosine residues in deoxyribonucleic acid. Biochemistry. 1974 Jul 30;13(16):3405–3410. doi: 10.1021/bi00713a035. [DOI] [PubMed] [Google Scholar]
  21. Ljungquist S. A new endonuclease from Escherichia coli acting at apurinic sites in DNA. J Biol Chem. 1977 May 10;252(9):2808–2814. [PubMed] [Google Scholar]
  22. Makino F., Munakata N. Deoxyuridine residues in DNA of thymine-requiring Bacillus subtilis strains with defective N-glycosidase activity for uracil-containing DNA. J Bacteriol. 1978 Apr;134(1):24–29. doi: 10.1128/jb.134.1.24-29.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Olivera B. M. DNA intermediates at the Escherichia coli replication fork: effect of dUTP. Proc Natl Acad Sci U S A. 1978 Jan;75(1):238–242. doi: 10.1073/pnas.75.1.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Olivera R. M., Bonhoeffer E. Replication of Escherichia coli requires DNA polymerase I. Nature. 1974 Aug 9;250(5466):513–514. doi: 10.1038/250513a0. [DOI] [PubMed] [Google Scholar]
  25. Sekiguchi M., Hayakawa H., Makino F., Tanaka K., Okada Y. A human enzyme that liberates uracil from DNA. Biochem Biophys Res Commun. 1976 Nov 22;73(2):293–299. doi: 10.1016/0006-291x(76)90706-3. [DOI] [PubMed] [Google Scholar]
  26. Shapiro R., Braverman B., Louis J. B., Servis R. E. Nucleic acid reactivity and conformation. II. Reaction of cytosine and uracil with sodium bisulfite. J Biol Chem. 1973 Jun 10;248(11):4060–4064. [PubMed] [Google Scholar]
  27. Shapiro R., Klein R. S. The deamination of cytidine and cytosine by acidic buffer solutions. Mutagenic implications. Biochemistry. 1966 Jul;5(7):2358–2362. doi: 10.1021/bi00871a026. [DOI] [PubMed] [Google Scholar]
  28. Shapiro R., Yamaguchi H. Nucleic acid reactivity and conformation. I. Deamination of cytosine by nitrous acid. Biochim Biophys Acta. 1972 Nov 9;281(4):501–506. doi: 10.1016/0005-2787(72)90150-5. [DOI] [PubMed] [Google Scholar]
  29. Tamanoi F., Okazaki T. Uracil incorporation into nascent DNA of thymine-requiring mutant of Bacillus subtilis 168. Proc Natl Acad Sci U S A. 1978 May;75(5):2195–2199. doi: 10.1073/pnas.75.5.2195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tye B. K., Chien J., Lehman I. R., Duncan B. K., Warner H. R. Uracil incorporation: a source of pulse-labeled DNA fragments in the replication of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1978 Jan;75(1):233–237. doi: 10.1073/pnas.75.1.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tye B. K., Lehman I. R. Excision repair of uracil incorporated in DNA as a result of a defect in dUTPase. J Mol Biol. 1977 Dec 5;117(2):293–306. doi: 10.1016/0022-2836(77)90128-0. [DOI] [PubMed] [Google Scholar]
  32. Tye B. K., Nyman P. O., Lehman I. R. Excision repair of uracil during replication of phiX174 DNA in vitro. Biochem Biophys Res Commun. 1978 May 30;82(2):434–441. doi: 10.1016/0006-291x(78)90894-x. [DOI] [PubMed] [Google Scholar]
  33. Tye B. K., Nyman P. O., Lehman I. R., Hochhauser S., Weiss B. Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Proc Natl Acad Sci U S A. 1977 Jan;74(1):154–157. doi: 10.1073/pnas.74.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Ullman J. S., McCarthy B. J. Alkali deamination of cytosine residues in DNA. Biochim Biophys Acta. 1973 Feb 4;294(1):396–404. doi: 10.1016/0005-2787(73)90094-4. [DOI] [PubMed] [Google Scholar]

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