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. 1983 Feb;153(2):652–657. doi: 10.1128/jb.153.2.652-657.1983

Genetic transformation of obligately chemolithotrophic thiobacilli.

S A Yankofsky, R Gurevich, N Grimland, A A Stark
PMCID: PMC221681  PMID: 6571832

Abstract

Genetic transformation of Thiobacaillus thioparus auxotrophs to prototrophy was obtained at frequencies of up to 10(-2) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent strain of the same bacterium. The rate at which transformation occurred depended on recipient growth rate and could be drastically reduced by depriving otherwise competent cells of either nitrogen or exogenous energy substrate. Interspecies marker transfer was also shown among several obligately chemolithotrophic members of the genus.

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Selected References

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