Abstract
Genetic transformation of Thiobacaillus thioparus auxotrophs to prototrophy was obtained at frequencies of up to 10(-2) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent strain of the same bacterium. The rate at which transformation occurred depended on recipient growth rate and could be drastically reduced by depriving otherwise competent cells of either nitrogen or exogenous energy substrate. Interspecies marker transfer was also shown among several obligately chemolithotrophic members of the genus.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cato A., Jr, Guild W. R. Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model. J Mol Biol. 1968 Oct 14;37(1):157–178. doi: 10.1016/0022-2836(68)90080-6. [DOI] [PubMed] [Google Scholar]
- Dougherty T. J., Asmus A., Tomasz A. Specificity of DNA uptake in genetic transformation of gonococci. Biochem Biophys Res Commun. 1979 Jan 15;86(1):97–104. doi: 10.1016/0006-291x(79)90386-3. [DOI] [PubMed] [Google Scholar]
- Kelly D. P. Influence of amino acids and organic antimetabolites on growth and biosynthesis of the chemoautotroph Thiobacillus neapolitanus strain C. Arch Mikrobiol. 1967 Feb 20;56(2):91–105. doi: 10.1007/BF00408761. [DOI] [PubMed] [Google Scholar]
- Kubinski Z. O., Kubinski H. Alterations in Bacillus subtilis transforming DNA induced by beta-propiolactone and 1,3-propane sultone, two mutagenic and carcinogenic alkylating agents. J Bacteriol. 1978 Dec;136(3):854–866. doi: 10.1128/jb.136.3.854-866.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lederberg E. M., Cohen S. N. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974 Sep;119(3):1072–1074. doi: 10.1128/jb.119.3.1072-1074.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lefevre J. C., Claverys J. P., Sicard A. M. Donor deoxyribonucleic acid length and marker effect in pneumococcal transformation. J Bacteriol. 1979 Apr;138(1):80–86. doi: 10.1128/jb.138.1.80-86.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Littauer U. Z., Yankofsky S. A., Novogrodsky A., Bursztyn H., Galenter Y., Katchalski E. Synthesis of peptidyl aminoacyl transfer RNA: a chemical method for the purification of transfer RNA's. Biochim Biophys Acta. 1969 Nov 19;195(1):29–49. doi: 10.1016/0005-2787(69)90600-5. [DOI] [PubMed] [Google Scholar]
- Matin A., Konings W. N., Kuenen J. G., Emmens M. Active transport of amino acids by membrane vesicles of Thiobacillus neapolitanus. J Gen Microbiol. 1974 Aug;83(2):311–318. doi: 10.1099/00221287-83-2-311. [DOI] [PubMed] [Google Scholar]
- Matin A. Organic nutrition of chemolithotrophic bacteria. Annu Rev Microbiol. 1978;32:433–468. doi: 10.1146/annurev.mi.32.100178.002245. [DOI] [PubMed] [Google Scholar]
- Peck H. D., Jr Energy-coupling mechanisms in chemolithotrophic bacteria. Annu Rev Microbiol. 1968;22:489–518. doi: 10.1146/annurev.mi.22.100168.002421. [DOI] [PubMed] [Google Scholar]
- Porter R. D., Guild W. R. Transfection in pneumococcus: single-strand intermediates in the formation of infective centers. J Virol. 1978 Jan;25(1):60–72. doi: 10.1128/jvi.25.1.60-72.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
- Stark A. A., Yankofsky S. A. Active transport of amino acids in Thiobacillus thioparus is a low-affinity process. J Bacteriol. 1981 Dec;148(3):956–965. doi: 10.1128/jb.148.3.956-965.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stark A. A., Yankofsky S. A. Regulation of amino acid transport in Thiobacillus thioparus. J Bacteriol. 1981 Dec;148(3):966–972. doi: 10.1128/jb.148.3.966-972.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinhart W. L., Herriott R. M. Fate of recipient deoxyribonucleic acid during transformation in Haemophilus influenzae. J Bacteriol. 1968 Nov;96(5):1718–1724. doi: 10.1128/jb.96.5.1718-1724.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor B. F., Hoare D. S. New facultative Thiobacillus and a reevaluation of the heterotrophic potential of Thiobacillus novellus. J Bacteriol. 1969 Oct;100(1):487–497. doi: 10.1128/jb.100.1.487-497.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VISHNIAC W., SANTER M. The thiobacilli. Bacteriol Rev. 1957 Sep;21(3):195–213. doi: 10.1128/br.21.3.195-213.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]